abcg2基因多态性与壮族肺结核患者抗结核药物性肝损伤的相关性.doc

ABCG2基因多态性与壮族肺结核患者抗结核药物性肝损伤的相关性分析 周泽文，马迎教1，柳智豪2，高建华2，许建国3，覃后继3 1 右江民族医学院公共卫生与管理学院，广西，百色，533000, 2 百色市疾病预防控制中心，广西，百色，533000, 3右江民族医学院附属医院呼吸内科 广西，百色，533000 【摘要】目的 探讨ATP-结合盒（ATP-binding cassette, ABC）转运蛋白家族成员ABCG2基因多态性与壮族肺结核（Tuberculosis, TB）初治患者抗肺结核药物性肝损伤的相关性。方法 选取2010年8月至2014年6月间百色市疾病预防控制中心诊治的58例壮族肺TB感染初治药物性肝损伤患者为研究对象，采用1:1匹配的方式设置对照组。采用问卷调查方式获取患者的一般临床信息；聚合酶链反应-限制性片段长度多态性法（Poylmeares Chin Reaction Restriction Fragment Length Poylmorphism, PCR-RFLP）检测ABCG2基因G34A（rs2231137）和C421A（rs2231142）位点基因型及分布频率。结果 ABCG2基因G34A和C421A位点均存在3种基因型。其中观察组G34A位点在G等位基因的频率为82.76%，明显高于对照组的65.52%；C421A位点观察组C等位基因的频率为79.31%，显著高于对照组的60.34%，但两组间比较差异有统计学意义（P0.05）。而且，G34A和C421A位点在观察组和对照组中的分布结果均显示两组间两个位点的基因型比较差异有统计学意义（P0.05）。 结论ABCG2基因多态性可能与壮族肺TB初治患者抗结核药物性肝损伤的发生存在显著性关联。 【关键词】肺结核；基因多态性；ABCG2；抗结核药物 Title Correlation of ABCG2 gene polymorphism and the anti tuberculosis drug-induced liver disease in Zhuang Lung TB patients Zhou Zewen1, Ma Yingjiao1, Liu Zhihao2, Gao Jianhua2, Xu Jianguo3, Qin following3 1 School of Public Health and Management, Youjiang Medical University for Nationalities Baise Guangxi 533000，China 2 Baise Center for Disease Control and Prevention, Guangxi Baise,533000,China； 3 Department of Respiratory Disease, the Affiliated Hospital of Youjiang Medical University for Nationalities，Baise Guangxi 533000，China 【Abstract】Objective To investigate the correlation of ATP-binding cassette (ATP-binding cassette, ABC) transporter family members of the ABCG2 gene polymorphism and the anti tuberculosis drug-induced liver disease in Zhuang Lung TB patients. Methods The 58 hospitalized anti tuberculosis drug-induced liver disease of firstly lung TB infection Zhuang patients were recruited from 2010 August to 2014 June in Affiliated Hospital of Youjiang Nationalities Medical College. 1:1 matching way was used for setting the control group. Questionnaire survey was used to obtain the general clinical information of patients; polymerase chain reaction - restriction fragment length polymorphism m