dcn基因转染对裸鼠胃癌移植瘤生长的影响-第三军医大学学报.doc

Decorin基因转染对人胃癌裸鼠移植瘤生长的影响 刘波王川胡在敏季文菜璨亢渝俊姜政 (重庆医科大学附属第一医院消化内科，重庆 400016) 探讨核心蛋白聚糖（Decorin，DCN)基因转染对人胃癌细胞SGC-7901裸鼠移植瘤生长及肿瘤血管生成的影响。构建裸鼠胃癌移植瘤模型，将重组质粒PEGFP-N1-DCN注入肿瘤中央及基底部，以空质粒组和生理盐水组作为对照，测量肿瘤体积和质量的变化，RT-PCR及Western blot检测DCN的表达差异，免疫组织化学染色方法检测血管内皮生长因子(Vascular Endothelial Growth Factor，VEGF)的表达差异。裸鼠胃癌移植瘤模型构建成功，与对照组比较，重组质粒组肿瘤生长速度减慢，肿瘤体积和质量明显变小(P0.05)；RT-PCR显示重组质粒组DCN表达明显增强，Western blot显示重组质粒组有融合蛋白的表达；免疫组化显示重组质粒组VEGF表达降低（P0.05)。DCN基因可以抑制裸鼠移植瘤生长，抑制肿瘤新生血管形成可能是其抗肿瘤作用的机制之一。 核心蛋白聚糖；转染；胃癌；血管内皮生长因子【中图分类号】R735.2 【文献标识码】Effect of decorin gene transfection on human gastric carcinoma xenograft growth in nude mice Liu Bo，Wang Chuan， Hu Zaimin，Ji Wen，Cai Can， Kang Yujun，Jiang Zheng (Department Of Gastroenterology,the First Affiliated Hospital Of Chongqing Medical University,Chongqing 400016) [Abstract] Objective: To investigate the effect of decorin gene transfection on the growth and angiogenesis of nude mice xenograft which bearing human gastric carcinoma cell SGC-7901. Methods: Gastric carcinoma xenograft model in nude mice was established, recombinant plasmid PEGFP-N1-DCN was injected to the center and bottom of the carcinoma, empty plasmid group and physiological saline group as control groups, the change of carcinoma volume and weight was measured, the different expression of DCN was detected by RT-PCR and western blot, the different expression of vascular endothelial growth factor (VEGF) was assessed by immunohistochemistry. Results: Gastric carcinoma xenograft model in nude mice was constructed successfully, compared with the control groups, slower growth speed, smaller volume and lighter weight was found in recombinant plasmid group (P0.05), RT-PCR showed that the expression of decorin gene in recombinant plasmid group was significantly higher than control groups, western blot indicated that the expression of fusion protein was found in recombinant plasmid group, immunohistochemistry showed that the expression of VEGF was down-regulated in rec