Platinum Taq DNA聚合酶说明书 【货号】 【规格】.pdfVIP

Platinum® Taq DNA C10966-018 100 C10966-026 250 C10966-034 500 C10966-083 5,000 5U/μl -20°C Platinum® Taq DNATaq PCR94°CTaq DNA 1-3PCR PCRPCRPCR PCR Taq DNA10342-053Platinum® Taq DNA5/μl PCR 100 250 500 5,000 Platinum® Taq DNA 20μl 50μl 100μl 1000μl 10×PCRMg 1.25ml 1.25ml 2.5ml 50ml 50 mM MgCl2 1ml 1ml 1ml 10ml 20 mM Tris-HCl pH 8.040 mM NaCl2 mM 0.1 mM EDTA1 mM DTT50% v/v 10×PCR 200 mM Tris-HCl pH 8.4500 mM KCl ® DNAPlatinum Taq DNATaq DNA ® Platinum Taq DNA Platinum®Taq DNA74°C3010nmoledNTP PCR PCRDNA, DNAPCR DNAPCR Platinum® Taq DNAPCR ® Platinum Taq DNAMgCl DNA 2 10.5ml 10×PCRMg 5μl 1× 10mM dNTP 1μl 0.2mM 50mM MgCl2 1.5μl 1.5mM 10μM 1.5μl 0.2μM DNA ≥1μl Platinum® Taq DNA 0.2μl 1.0* 50μl 50μl N/A * 1.02.5 250μl 3 4PCR94°C302 525-35 94°C30 55°C30 72°C1kb1 6PCR4°C-20°C 7 1. Chou, Q., et al.(1992) Nucl. Acids Res. 20 , 1717. 2. Sharkey, D.J., et al. (1994) BioTechnology 12 , 506. 3. Westfall, B.A., et al. (1997) Focus® 1