米曲霉胞链接与发酵液中蛋白动态变化的研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.8 米曲霉发酵液中蛋白动态变化的研究 冯广莉，刘洪伟，许喜林，李晓凤 （华南理工大学轻工与食品学院，广东广州510640） 摘要：本文利用葡萄糖和吐温80 作为额外碳源培养米曲霉，对其生物量积累，胞链接蛋白以及发酵液蛋白随培养时间的变化情 况进行了研究。以葡萄糖作为额外碳源时，米曲霉生物量积累速度较快，在培养24 h 已达最大值，为31.97 g/L ；当米曲霉以吐温80 作为额外碳源时菌体积累较慢，在96 h 时达到最大值，为11.15 g/L。发酵液中蛋白总量在以葡萄糖作为额外碳源时比以吐温80 时低。 以吐温80 作为额外碳源时，胞链结蛋白在72 h 达到最大值的5.65 mg/g，当以葡萄糖作为额外碳源时，米曲霉胞链结蛋白含量在48 h 达到最大值，为1.71 mg/g。利用SDS检测 192 h 内胞连接蛋白质的表达与发酵液中的蛋白质的含量变化与利用lowry 法测定 的蛋白质含量具有一致的变化趋势：在米曲霉的快速生长期和稳定期时胞链结蛋白种类增加，在衰退期胞链结蛋白种类减少。 关键词：米曲霉；蛋白质表达；SDS图谱；胞链接；发酵 文章篇号：1673-9078(2013)8-1832-1836 Expression Changes of Proteins in Fermentation Broth of Aspergillus oryzae FENG Guang-li, LIU Hong-wei, XU Xi-lin, LI Xiao-feng (School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: In this paper, biomass accumulation, dynamic expression of proteins in fermentation broth of Aspergillus oryzae were studied when glucose or tween 80 were used as additional carbon sources. When glucose was used as additional carbon source, biomass accumulation of A.oryzae was fast er than tween 80 and reached the maximum of 31.97 g/L at 24 h, but accumulation of A.ryzae was slow when tween 80 was used, which reached the maximum of 11.15 g/L at 96 h. Total proteins in fermentation broth was higher with glucose as additional carbon source. Cell-bound proteins reached the maximum value of 5.65 mg/g at 72 h with tween 80 as additional carbon source, while cell -bound proteins reached the maximum of 1.71 mg/g at 48 h with glucose as additional carbon source. Expression of cell-bound proteins detected by SDS within 192 h showed that cell-bound proteins increassed at the rapid growth and stationary phages , and redu