组织工程软骨和柚皮苷联合修复兔膝关节软骨缺损组织学证实.docVIP

组织工程软骨与柚皮苷联合修复兔膝关节软骨缺损的组织学证实 李春根1，曲? 弋1，叶? 超1，陈? 婧2，王逢贤1，李鹏洋1，李素华1，任敬佩1，亓? 建1 北京中医药大学东直门医院，1骨科，2脑病科，北京市? 100700 Histology research on repairing of rabbit articular cartilage defects with naringin and tissue engineering cartilage Li Chun-gen1, Qu Yi1, Ye Chao1, Chen Jing2, Wang Feng-xian1, Li Peng-yang1, Li Su-hua1, Ren Jing-pei1, Qi Jian1 1Department of Orthopedics, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China; 2Department of Neurology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China 摘要? 背景：目前临床上虽有多种方法用于治疗软骨缺损，但没有从根本上解决关节软骨缺损修复问题。?目的：通过组织学研究进一步评价柚皮苷结合组织工程软骨修复兔关节软骨缺损的效果。?方法：取兔骨髓间充质干细胞体外增殖后，复合于改建后的脱细胞真皮基质载体上，制成组织工程软骨，植入到兔膝关节软骨缺损，并以柚皮苷汤灌胃，于 4，8周后分别对修复组织进行苏木精-伊红、Masson三色染色、甲苯胺蓝染色、Ⅱ型胶原染色、Ⅹ型胶原染色等组织学检查。?结果与结论：术后8周, 柚皮苷结合干细胞复合体组缺损处修复组织变成乳白色，半透明光滑组织，缺损修复组织与周围正常软骨已基本难区分，表面光滑。组织学检查发现修复缺损处基本为新生软骨填充。结果证实，柚皮苷结合组织工程软骨能提高家兔膝关节软骨缺损的修复质量。 HYPERLINK / 中国组织工程研究杂志出版内容重点：组织构建； HYPERLINK / 骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞； HYPERLINK / 骨质疏松； HYPERLINK / 组织工程 全文链接： 关键词?：? HYPERLINK /CN/abstract/abstract8418.shtml 组织构建；? HYPERLINK /CN/abstract/abstract8418.shtml 软骨组织工程；? HYPERLINK /CN/abstract/abstract8418.shtml 软骨缺损；? HYPERLINK /CN/abstract/abstract8418.shtml 骨髓间充质干细胞；? HYPERLINK /CN/abstract/abstract8418.shtml 脱细胞真皮基质；? HYPERLINK /CN/abstract/abstract8418.shtml 组织工程软骨；? HYPERLINK /CN/abstract/abstract8418.shtml 柚皮苷；? HYPERLINK /CN/abstract/abstract8418.shtml 兔； ?? Abstract： BACKGROUND: At present, there are many methods to treat cartilage defects, but none radically repairs the articular cartilage defects.?OBJECTIVE: To histologically verify the effect of naringin combined with tissue engineering cartilage on the repair of rabbit articular cartilage defects.?METHODS: Rabbit bone marrow mesenchymal stem cells following in vitro proliferation were compounded onto acellular dermal matrix, which was then implanted into rabbit knee cartilage defects. Naringin was also given by lavage. Hematoxyli