卡托普利对成骨细胞增殖及ⅰ型胶原基因表达水平的影响.doc

因你的系统上传不了大的文件，我已发邮件到你的邮箱了 甘草酸对大鼠骨髓间充质干细胞体外成骨分化的影响 [摘要] 目的：观察对体外培养影响。方法：双抗体两步夹心酶联免疫吸附法检测对细胞骨钙素的影响10-6mol.L-1浓度组作用7d时效果明显。结论：甘草酸能促进大鼠骨髓间充质干细胞向成骨细胞方向分化。 [关键词] ；成骨细胞；lycyrrhizic acid on proliferation and differentiation of rat bone mesenchymal stem cells in vitro CHEN Qiusheng1 ，LI Jin2，ZHOU Rumang2，YAN Wejiang2，LIU Yuyu2 (1 Laboratory of Physiolological Sciences, Guangdong Medical College,, 2 Dept of Pharmacology, Guangdong Medical College,guandong , Zhanjiang 524023) [Abstract] Objective: To observe the effect of glycyrrhizic acid on cultured human bone marrow mesenchymal stem cell alkaline phosphatase and osteocalcin expression. Methods: The isolation and purification of rat bone marrow mesenchymal stem cells from the paste method for screening the wall. PNPP method was used to study the effects of glycyrrhizic acid on the differentiation, Elisa was used to detect osteocalcin secretion of bone marrow mesenchymal stem cells. Results: The passage pastes the method screening wall can get bone marrow stromal cells, which are mesenchymal stem cells, were induced to differentiate into osteoblasts. Glycyrrhizic acid increased rat bone marrow mesenchymal stem cells after osteogenic induction of alkaline phosphatase in cells with 10-6 mol.L-1 resveratrol for 7 days. Conclusion: Glycyrrhizic acid can promote rat bone marrow mesenchymal stem cells to differentiate into osteoblasts. [Key words] glycyrrhizic acid; bone marrow mesenchymal stem cells; osteoblasts; proliferation; differentiation 甘草（Glycyrrhiza）为豆科植物甘草、胀果甘草和光果甘草的根及根茎，始载于《神农本草经》，被列为上品，传统中医药认为它具有补脾益气，清热祛痰等功效。甘草的化学成分较复杂，其中甘草酸(Glycyrrhizic Acid，GA)是最重要的活性成分，具有抗炎抗过敏、抗病毒、免疫调节作用、保肝、协同促进吸收等功能。研究表明，甘草的干燥根及根茎以及其提取物甘草酸以及其他甘草提取物都有较好的防治骨质疏松症的作用。我们课题组前期的预试也发现GA和雌激素联合应用可增加骨羟脯氨酸的含量，增大骨断裂载荷和最大载荷，改善去卵巢小鼠骨的结构，提升松质骨结构力学，但目前甘草酸对骨髓间充质干细胞成骨分化作用的研究较少，本实验观察对增殖影响，为用防治骨质疏松提供更多的实验依据。 1 材料低糖培养基DMEM高糖培养基、四硝基苯基磷酸二钠盐(PNPP)标准品；ELISA试剂盒NAPCO二氧化碳恒温培养箱（5420-1， Precision Scientific美国）；XD-101倒置相差显微镜（江南光电股份有限公司）；2 方法 2.1的制备及培养 一月龄SD大鼠10只，雌雄各半，体重70g+1