2. 非常重要 qPDH1 locus associated with pod dehiscence in soybean.pdfVIP

Mol Breeding (2010) 25:407–418 DOI 10.1007/s11032-009-9340-5 Fine mapping and development of DNA markers for the qPDH1 locus associated with pod dehiscence in soybean Masaya Suzuki • Kaien Fujino • Yumi Nakamoto • Masao Ishimoto • Hideyuki Funatsuki Received: 3 August 2009 / Accepted: 21 September 2009 / Published online: 6 October 2009 Springer Science+Business Media B.V. 2009 Abstract Pod dehiscence (shattering) is a major confined the location of qPDH1 to a 134-kb region on cause of yield loss in mechanical harvesting of chromosome 16 (formerly linkage group J), where soybeans. To develop useful selection markers, we ten putative genes were predicted to be present. None conducted a high-resolution mapping of a major of these genes showed significant sequence homology quantitative trait locus (QTL) controlling pod dehis- with the Arabidopsis genes that have previously been cence, designated as qPDH1. The progeny of a reported to be associated with pod dehiscence, residual heterozygous line, which was a recombinant suggesting the presence of a novel gene and mech- inbred line segregating only for the genomic region anism underlying pod dehiscence in soybean. around qPDH1, was screened for flanking markers to Sequencing analysis of the parental shattering-resis- obtain various recombinants in the vicinity of the tant and -susceptible cultivars for the candidate genes QTL. Analysis of the relationship between degree of revealed a high-frequency nucleotide polymorphism pod dehiscence and graphical genotype of these lines in this genomic region between the cultivars. Three markers were developed using