烟草黑胫病菌厚垣孢子和菌丝体在土壤中的存活-南京农业大学学报.PDF

烟草黑胫病菌厚垣孢子和菌丝体在土壤中的存活-南京农业大学学报.PDF

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烟草黑胫病菌厚垣孢子和菌丝体在土壤中的存活-南京农业大学学报

1998,21( 1) : 4145 J ournal of N anj ing A g ric ult ur al Un iv er sity 1 1 1 2 王 革 郑小波 陆家云 李天飞 1 ( , 210095 2 ) 40%70% , 4 25 ,: 8 ,, 2 3 , 3 ,, ; ; ; S435.72 Survival of chlamydospores and mycelia of Phytophthora nicotianae in soil 1 1 1 2 Wang e , Zheng Xiaobo , Lu Jiayun and Li T ianfei 1 ( Key Lab of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, 2 Nanjing Agric Univ, Nanjing 210095; Yunnan Tobacco Research Institute) ABSTRACTSuspension of chlamydospores and mycelia of P hy top hthora n icot ianae prepared under labora- tory conditions were mixed into the sterilized soil samples with 40%, 70% water content and maintained at 4 , 25 and room temperature respectively Survival of chlamydospores and mycelia were tested peri-. odically. T he results showed the chlamydospores could survive at least 8months in soil, with more thicker wall and contracted protoplasm . How -ever the mycelia could only survive for 2 months. The chlamy- dospores in soil within 3 months could be detected by the method of selective medium directly. After 3 months the baiting method must be applied. Between the effect of temperature and water content under which soil samples maintained the effect of temperature on the surrival of chlamydospores and mycelia, was more striking. Low temperature was beneficial for their survival. T he effect of different water content on their survival was not obvious. Key wordstobacco black shank; P h

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