hplc法同时测定丹参类药材中水溶性活性成分的含量（Simultaneous determination of water-soluble active components in Radix Salviae Miltiorrhizae by HPLC）.docVIP

hplc法同时测定丹参类药材中水溶性活性成分的含量（Simultaneous determination of water-soluble active components in Radix Salviae Miltiorrhizae by HPLC） The fallen flower, in gloomy mood, Speechless blame dongfeng. By cutting out the superfluous Qiu tree, marked differences from the new February flowers. He is resolute. To cross the Yellow River ice jam Sichuan, will climb the Taihang mountains snow mountain. All alone in a foreign land, during the festive season. Simultaneous determination of water-soluble active components in Radix Salviae Miltiorrhizae by HPLC Update Date: 12-21 [Abstract] Objective To establish a HPLC method for simultaneous determination of Salvia miltiorrhiza and Gansu Salvia Miltrorrhiza method of Protocatechualdehyde and salvianolic acid B content of sodium, ginseng, through 3 kinds of water soluble active ingredients determination, evaluation of the quality of wild and cultivated in Gansu, Gansu wild and cultivated Salvia salvia. Methods the chromatographic column was C18 chromatographic column (4.6 mm * 250 mm, 5 m); the mobile phase was methanol acetic acid water (20 W 80 W 1) with flow velocity of 1 mL/min. Two yuan gradient elution. Results the sodium chloride, protocatechuic aldehyde and salvianolic acid B had good internal relations at 58~1455 ng, 15~162 ng and 825~8250 ng respectively, and the recoveries were 102.20%, 101.53% and 103.03% respectively. Conclusion the method is simple, accurate and has a good separation effect. It can be used as a quantitative method for simultaneous determination of 3 water-soluble components in Salvia miltiorrhiza bge. Keywords high performance liquid chromatography (HPLC); Radix Salviae Miltiorrhizae; Gansu Danshen root; water soluble component; Danshensu Sodium; protocatechuic aldehyde; salvianolic acid B; cultivation; wild; Pharmacopoeia contained Chinese Danshen Salvia miltiorrhiza Salvia miltiorrhiza Bge [1], located in Gansu and Tianshui area of Longnan, the only resources without goods, in recent years, Qingyang, Ping