新型组织工程纤维环支架的制备及其体外性能研究 - 第三军医大学学报.docVIP

聚乳酸-聚己内酯组织工程纤维环支架的制备及其性能研究 李玉东1, 徐源1,周强1,宋磊1,刘威2,甘翼博1,李培1,李松涛1,莫秀梅2 （400038 重庆，第三军医大学西南医院骨科，国家与地方联合组织工程实验室1；201620上海，东华大学化工与生物工程学院2） [摘要] 目的 探讨新的静电纺丝方法制备聚乳酸-聚己内酯(P(LLA-CL))纳米纱作为组织工程纤维环支架的可行性，检测其理化性能及细胞相容性。方法 在传统静电纺丝方法制备P(LLA-CL) 纳米纤维支架的基础上，改用动态水流接收装置制备相同成分的纳米纱支架，并对材料的表面形态、纤维直径、孔径、孔隙率、力学性能进行评估，分离培养兔纤维环细胞种植到支架上，观察细胞在材料上的生长形态、粘附、增殖、渗透情况。结果 P(LLA-CL)纳米纤维支架表面呈致密的网状结构，纤维排列无方向性，而P(LLA-CL)纳米纱支架表面呈疏松的网状结构，纤维排列有一定的取向；纳米纤维支架的孔径为(4.57±1.87)μm，孔隙率(74.2±1.5)%，纳米纱的孔径为(38.43±15.54)μm，孔隙率(86.7±9.3)%；两者的力学性能相似。扫描电镜（SEM）显示纤维环细胞在纳米纤维支架上呈多边形生长，在纳米纱支架上呈长梭形生长；CCK-8结果显示细胞在两种支架材料上的粘附均很好，细胞在纳米纱上的增殖较纳米纤维支架明显增加；HE染色提示细胞只能生长在纳米纤维支架表面，而能很好的渗透到纳米纱支架内部。结论 静电纺丝方法制备的P(LLA-CL)纳米纱支架具有良好的三维孔隙结构和细胞相容性，有望成为较理想的组织工程纤维环支架。 [关键词] 纤维环；组织工程；支架；聚乳酸-聚己内酯； 纳米纱 [中图法分类号] [文献标志码] A Fabrication and analysis of a novel tissue engineered scaffold for annulus fibrosus Li Yudong1,Xu Yuan1,Zhou Qiang1,Song Lei1,Liu Wei2, Gan Yibo1,Li Pei1, Li Songtao1,Mo Xiumei2 （1 National Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China；2 College of Chemistry and Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, China） [Abstract] Objective To investigate the feasibility of Poly(L-lactide-co-caprolactone) (P(LLA-CL))nanoyarn prepared by a novel electrospinning method as annulus fibrous tissue engineering scaffold, further tests to evaluate the physicochemical properties and cytocompatibility of the scaffold. Methods On the basis of traditional electrospinning method which used to prepare P(LLA-CL) nanofiber scaffold, a novel P(LLA-CL) nanoyarn scaffolds were prepared by dynamic liquid electrospinning. These scaffolds were characterized in terms of fiber morphology, fiber diameter, pore size, porosity, and mechanical properties, then rabbit annulus fibrous (AF) cell were isolated and seeded on the scaffolds. Cell morphology, attachment,