大鼠重症急性胰腺炎脑损伤脑内核转录因子的表达.pdfVIP

江苏医药2008年1月第34卷第1期 Jian~su Med J，January 2008，Vol 34，No．1 · 论著 · 大鼠重症急性胰腺炎脑损伤脑内核转录 因子的表达 袁伟燕 黄中伟 黄 华 施公胜 【摘要】 目的 研究大鼠重症急性胰腺炎(SAP)时脑组织海马区域核转录因子KB p65(NF-~cB p65)的表达及其与脑损伤之间关系。方法 5 牛磺胆酸钠逆行注入大鼠胰胆管建立SAP模型，尼 氏染色检测脑组织海马区神经元损伤情况，免疫组化法及逆转录聚合酶链反应(RT-PCR)法检测海 马组织NF-KB p65的表达。结果 在3、6、12 h三个时间点NF-~cB p65 mRNA的半定量结果，SAP 组0．63±0．05、1．05±0．06和0．92±0．05，明显高于生理盐水组0．114-0．01、0．12±0．01和0．084- 0．01(P0．05)。结论 NF-~cB p65参与大鼠SAP 脑损伤的发生发展，在脑损伤的早、中期起作用。 【关键词】 大鼠；重症急性胰腺炎；脑损伤；海马；核转录因子KB 【中图分类号】 R657 【文献标识码】 A 【文章编号】 0253—3685(2008)01—0073—02 Expression of hippocampal nuclear factor-r,B in rats with severe acute pancreatitis accompanied with brain injury Weiyan， G Zhongwei，HUANG Hua，et a1．Department Emergency Medicine，A liated Hospitaf，Nantong University，Nantong 226001，a [Abstract] Objective To observe hippocampal nuclear factor kappa B p65(NF_JcB p65) expression in rats with severe acute pancreatitis(SAP)and its relationship with brain injury．Methods The SAP mode1 was induced by retrograde injection of 5 sodium taurocholate into biliopancreatic duct in rats．Nissle stain was used to evaluate the degree of the brain inj ury．NF-KB p65 was assa~red by immunohistochemistry and reverse transcriptase polymerase chain reaction(RT_PCR)．Results The half quantity of NF-KB p65 mRNA at the 3rd。6th and 12th hour in SAP group and NS group was 0．63±0．05 VS．0．1l±0．01，1．05±0．06 VS．0．12 4-0．01，and 0．92±0．05 VS．0．08±0．01， respectively(P~0．05)．The injury of hippocampal neurons was much severer in SAP 3，6，12 h groups than that in NS groups．Conclusion NF-KB p65 accelerates the brain injury in SAP rats，and affects its procession mainly during its earlier and middle periods． [Key wordsl Rat；Severe acute pancreatitis；Brain iNury；Hippocampus；Nuclear factor-~cB l Jiangsu Med J，January 2008，34(1)：73—74．1 重症急性胰腺炎(SAP)是临床常见急重症之