PI3K-AKT信号传导通路对鼻咽癌细胞增殖的影响.DOC

PI3K/AKT信号传导通路摘要目的 研究PI3K/AKT信号传导途径对人低分化鼻咽癌细胞系在CNE-2Z生长、增殖作用应用wortmannin抑制PI3K的活性，以绘制生长曲线、MTT观察wortmannin对CNE-2Z细胞生长、增殖的作用；以免疫细胞化学分别检测wortmannin处理培养后的CNE-2Z细胞中PI3K、AKT和bcl-2的表达，RT-PCR检测wortmannin处理后的细胞bcl-2 mRNA的表达情况，ELISA法检测wortmannin处理后的CNE-2Z细胞中的磷酸化的AKT的含量，流式细胞仪检测wortmannin处理培养后的CNE-2Z细胞的凋亡。结果wortmannin对CNE-2Z细胞生长、增殖具有抑制作用，而且呈剂量依赖性。wortmannin作用后CNE-2Z细胞的PI3K和bcl-2的蛋白表达均减弱,而AKT的表达无明显变化。揭示wortmannin可以抑制CNE-2Z细胞bcl-2 mRNA的表达。wortmannin能显著抑制磷酸化的AKT的表达，并呈一定的剂量依赖性。Wortmannin还可以诱导CNE-2Z细胞的凋亡PI3K的抑制剂wortmannin可以有效抑制鼻咽癌CNE-2Z细胞的生长和增殖，并且呈剂量依赖性；从基因和蛋白水平抑制CNE-2Z细胞中bcl-2的表达，诱导CNE-2Z细胞凋亡；同时使磷酸化的AKT的表达降低。表明wortmannin可以通过PI3K/AKT信号传导途径抑制鼻咽癌细胞的增殖，诱导鼻咽癌细胞的凋亡。PI3K/AKT途径可能是鼻咽癌细胞增殖和抗凋亡的重要信号传导通路。 [] PI3K；AKT；信号传导通路；鼻咽癌细胞；增殖 [Abstract] Objective To investigate the effects of the PI3K/AKT pathway on growth，proliferation on CNE-2Z cells. MethodsPI3K activity was inhibited by wortmannin(WT). Cells growth curves and MTT assay were used to identify the effect of the wortmannin（WT）on growth and proliferation in CNE-2Z cells. AKT, PI3Kprotein in CNE-2Z cells treated with the wortmannin were detected by IHC. P-AKT was evaluated by ELASA. Apoptotic features in CNE-2Z cells treated with the wortmannin were analyzed by FCM. Results The growth and proliferation of CNE-2Z cells were inhibited significantly by wortmannin compared with the control group, which was in dose dependent manner. The expression of PI3Kin the group which 作者姓名：龚晓萌 地址：安徽省蚌埠市治淮路800号蚌埠医学院病理学教研室 邮编233000 电话 中级职称 Email gongxmeng@163.com were treated with wortmannin was lower than that in the control group. However, expression of AKT was no significant decrease. The expression of P-AKT reduced significantly compared with the control group，which was in dose dependent manner. Conclusions wortmannin could inhibit the growth and proliferation of CNE-2Z cells PI3K/AKT signaling pathway might be involved in proliferation of CNE-2Z cells. It was necessary to continue more research on wortmanin. Inhibiting PI3K/AKT sig