蒲黄总黄酮对犬急性心肌缺血的保护作用-中国试验方剂学杂志.DOC

龙眼参黄酮对犬急性心肌缺血时心外膜电图及心肌酶的影响 赵宏1，杨煜2，王景祥1，吕文伟2* （1..吉林省柳河医院, 吉林 柳河 135300；2.吉林大学白求恩医学院，吉林 长春 130021） [摘 要] 目的:研究龙眼参黄酮对犬急性心肌缺血时心外膜电图及心肌酶的影响。方法:采用麻醉开胸犬结扎左冠状动脉前降支（LAD）制备急性心肌缺血模型，观察龙眼参黄酮对心外膜电图（EECG）和心肌酶的影响。结果: 龙眼参黄酮可减少缺血程度(∑-ST)，降低缺血范围(N-ST)，降低血清中磷酸肌酸激酶（CPK）、乳酸脱氢酶（LDH）的活性，降低血清中游离脂肪酸（FFA）、过氧化脂质（LPO）含量，提高超氧化物歧化酶（SOD）、谷胱甘肽过氧化物（GSH-Px）、一氧化氮（NO）, 一氧化氮合酶（NOS）的活性。结论: 龙眼参黄酮对实验性心肌缺血犬心肌具有保护作用。 [关键词] 龙眼参黄酮;心肌缺血; 心外膜电图; 心肌酶 [中图分类号] [文献标识码] B [文章编号] Effects of Longyanshen flavonoids on epicardial electrogram and myocardial enzymes in acute myocardial ischemic dogs ZHAO Hong 1 , YANG Yu 2 ,WANG Jing-xiang1，LU Wen-ei2* (1. Liuhe hospital, Jilin , Liuhe 135300; 2. Department of Pharmacology , School of Basic Medical Sciences , Jilin University , Changchun 13002 ) [Abstract]Objective: To investigate the effects of Longyanshen flavonoids(LF) on epicardial electrogram （EECG）and myocardial enzymes in acute myocardial ischemic dogs. Method: We adopted anesthetized thoraco-opened dogs to establish the acute myocardial ischemia models by ligation of the left anterior descending branches，the influences of LF were measured by the changes of EECG and myocardial enzymes. Result: It was observed that LF could reduce the severity and area of myocardial ischemia (∑-ST and N-ST). Moreover，LF not only decreased the content of Free fatty acids （FFA） and LPO in serum, as well as the activity of CPK and Lactate dehydrogenase （LDH） in serum，but also improved the activity of SOD, Glutathione peroxidase （GSH-Px）,Nitric oxide （NO）and Nitric oxide synthase （NOS）.Conclusion: LF has a protective effect on myocardium in experimental myocardial ischemic dogs. [Key words] Longyanshen flavonoids（LF） ; myocardial ischemia; epicardial electrogram （EECG）; myocardial enzyme -------------------------------------------------------------------------------------------------------------- 收稿日期] [通讯作者] *吕文伟.（E-mai