基质金属蛋白酶-12及其组织抑制物-1在皮肤鳞状细胞癌中表达及意义.docVIP

基质金属蛋白酶-12及其组织抑制物-1在皮肤鳞状细胞癌中表达及意义[摘要]目的：检测基质金属蛋白酶-12(MMP-12)及其组织抑制物-1（TIMP-1）在皮肤鳞状细胞癌（SCC）中的表达并探讨其与肿瘤分化、淋巴转移的关系。方法：免疫组化法检测MMP-12及TIMP-1在20例正常皮肤组织和32例鲍温病（BD）、53例SCC患者皮损中的表达。结果：在正常人皮肤和BD患者皮损中，MMP-12不表达，TIMP-1呈弱阳性表达，而两者在皮肤SCC患者皮损中均呈弥漫表达，MMP-12在SCC患者瘤组织中表达阳性率为92.4%，在低分化组或有淋巴结转移组中的表达明显高于高分化组（P＝0.009）或无淋巴结转移组（P＝0.01）；TIMP-1在SCC患者皮损中表达阳性率为84.9%，在高分化组或无淋巴结转移组中的表达明显高于低分化组（P＝0.005）或有淋巴结转移组（P＝0.01）。结论：MMP-12、TIMP-1蛋白的表达可能与SCC的癌分化和转移有关，MMP-12高表达与TIMP-1低表达提示SCC肿瘤细胞可能有较高的侵袭潜能。 [关键词]鳞状细胞癌；Bowen病；巨噬细胞金属弹力酶；金属蛋白酶类组织抑制剂 [中图分类号]R739.5[文献标识码]A[文章编号]1008-6455（2011）02-0237-03 Expression of matrix metalloproteinase-12 and its tissue inhibitor-1 in cutaneous squamous cell carcinoma XUAN Min1,2,YANG Hui-lan1,FAN Jian-yong1,LIANG Jie1,ZHANG Ling1 (1.Department of Dermotology,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou 510010,Guangdong,China；2.Graduate School of Nanfang Medical University,Guangzhou 510015,Guangdong,China） Abstract:ObjectiveTo investigate the expression of matrix metalloproteinase-12（MMP-12）and its tissue inhibitor-1（TIMP-1）in cutaneous squamous cell carcinoma（SCC）and the relationship between the expression and tumor differentiation and lymph node metastasis.MethodsImmunohistochemical method was used to detect the expression of MMP-12 and TIMP-1 proteins on the paraffin embedded sections of 53 patients with SCC and 32 patients with BD.ResultsMMP-12 and TIMP-1 proteins were diffusely expressed in the tumor nets and mesenchymal cells around the nets,while in normal epidermis and BD MMP-12 was negative,and TIMP-1 was weakly positive. The expressive rate of MMP-12 was 92.4％,and the expression in poorly differentiated tumous or with lymph node metastasis was significantly higher than that in highly differentiated ones（P＝0.009）or without lymph node metastasis （P＝0.01）. The expressive rate of TIMP-1 was 84.9％,and the expression in highly differentiated tumous or without lymph node metastasis was significantly higher than that in poorly differe