促红细胞生成素对心肌梗死大鼠血流动力学及梗死面积的影响.docVIP

　　促红细胞生成素对心肌梗死大鼠血流动力学及梗死面积的影响 ：赵玉伟 李法琦 邱炯 周平 李万玉 黄妍 【摘要】 目的 探讨重组人促红细胞生成素(rhEPO)对大鼠急性心肌梗死(AMI)后24 h血流动力学和左心室梗死面积的影响。方法 24只雄性SD大鼠随机分为假手术组、AMI组和EPO组，结扎左冠状动脉前降支建大鼠AMI模型，EPO组同时给予rhEPO 5 000 U/kg腹腔内注射;24 h后记录大鼠血流动力学参数并 计算 左心室梗死区与左心室质量比值。结果 AMI组各项血流动力学参数均明显下降;EPO组血流动力学参数均明显改善(Plt;0.05，Plt;0.01)，尤其反映心肌舒张功能的指标改善更显著(Plt;0.01);EPO明显降低左心室梗死区与左心室质量比值(Plt;0.05)。结论 EPO急性干预缩小大鼠AMI后的梗死面积并明显改善其血流动力学参数，尤其对舒张功能改善更为明显。 【关键词】 促红细胞生成素;心肌梗死;血流动力状况;梗死面积;大鼠 　　【Abstract】 Objective To investigate the effect of erythropoietin (EPO) on the 24 hours hemodynamic status and infarct area of the left ventricle in rats after acute myocardial infarction (AMI). Methods 24 male SD rats : shamoperated, AMI and EPO groups. AMI rat model e time an EPO (rhEPO, 5 000 U/kg). The hemodynamic parameters including blood pressure (BP), left ventricular systolic pressure (LVSP), maximal rate of increase of ventricular pressure (dp/dtmax), left ventricular diastolic pressure (LVDP), maximal rate of decrease of ventricular pressure (dp/dtmin) and left ventricular end diastolic pressure (LVEDP) in rats 24 hours after operation ined. The size of infarct myocardial tissue and the ratio of the yocardial tissue as easured. Results pared operated group, all hemodynamic parameters of rats declined remarkably in AMI group. pared odynamic parameters of rats in EPO group proved in different degrees: LVSP and dp/dtmax related to cardiac systolic function proved but the differences in associated arkably improved (Plt;0.05 or Plt;0.01); mean arterial pressure (MAP) shoyocardial tissue in the left ventricle and the ratio of the yocardial tissue as eliorate the hemodynamic parameters in rats prove the cardiac diastolic function. Treatment of EPO can reduce the myocardial infarct area of AMI rats. 　　【Key odynamic status; Infarct area; Rat 　　促红细胞生成素(EPO)是由成人肾脏分泌的分子量为34 kD的调节红细胞生成的生长因子，它通过抗细胞凋亡的机制来促进红系祖细胞的增殖、分化和成熟。缺氧诱导因子1(HIF1)是由HIF1α和HIF1β两个亚单位组成的异二聚体，在缺氧应激状态下，HIF1的两个亚单位结合后进入细胞核，结合并激活位于EPO启动子上的缺氧反应元件，从而促进EPO的转录和表达。由于EPO