Ghrelin对3T3-L1前脂肪细胞增殖和分化的影响及相关机制探讨.PDF

11 1 中国当代儿科杂志 V o .l 11N o. 1 2009 1 C hin J C ontem p Pedia tr Jan. 2009 Ghrelin 3T3L1 刘静 林汉华 程佩萱 胡秀芬 卢慧玲 (华中科技大学同济医学院附属同济医院儿科 北 武汉 430030) [ ] ghre lin 3T 3L 1 3T3 L 1 M TT ghrelin RTPCR ghrelin cm yc mRNA ; ghre lin O RTPCR ( PPAR) CAAT / ( C / EBP) m RNA 10- 7 ~ 10- 15 m ol/ L ghrelin 24 h ghre lin cm ycm RNA ( P 0. 05) G hrelin ; PPAR C /E BPm RNA ( P 0. 05 ) ghrelin PPAR C / EBPm RNA 8 d 2 d (P 0. 0 1) G hre lin 3T3L 1G hre lin cm yc ; ghre lin PPARC / EBPmRNA [ 2009 11( 1) : 69- 73] [ ] G hre lin; ; ; ; [ ] R - 33 [ ] A [ ] 100 8- 8830 ( 2009) 01- 0069 - 05 Effects of ghrelin on th e proliferation and differen tiation of 3T3L 1 p readipocytes and its possible m echanism s L I U J ing, L IN H a nH ua, CH EN G P e iX ua n, H U X iuF en, L U H u iL ing. D ep artm ent of P ed iatrics, Tong i H osp ita l, Tong iM ed ica l Colleg e, H uazh ong Un ive rsity of S c ience a nd Technolog y, W uh an 4 30030, Ch ina (L inH H , Em a il: hh lin@ th. tm u. edu. cn) Ab strac t O bjec tive T o inv estig ate the effects o f gh re lin on the pro life ra tion and d ifferen tiation o f 3T 3L 1 preadipocyte and stu dy the possib le m ech an ism s. M ethod s 3T 3L 1 preadipocytes w ere cu ltured in v itro. The pro liferation po ten tia ls of 3T3L 1 pread ipocy tes th at w ere