组胺对荷黑色素瘤C57BL-6小鼠微血管壁通透性的影响.doc

　　组胺对荷黑色素瘤C57BL/6小鼠微血管壁通透性的影响 ：秦迎松 庞训雷 于红丽 【摘要】 目的 研究组胺对荷黑色素瘤C57BL/6小鼠各器官及肿瘤微血管壁通透性的影响，为通过组胺增加抗体靶向药物在肿瘤局部的富集提供依据。方法 细胞悬液接种法制备荷瘤模型，伊文思蓝法检测各器官及肿瘤微血管壁通透性，组织化学方法观察组织结构改变。结果 ①成功建立荷瘤模型，成瘤率100％。②对照组伊文思蓝（Evans blue, EB）在各器官分布达平衡的过程不同：肝、肺存在明显再分配；小肠、脾和肿瘤组织中EB随时间不断积累，在高水平上保持平衡；心、肾中EB含量随时间的积累过程比较缓慢，升高幅度不显著；脑组织中EB含量极少。③组胺组分布达平衡前各组织中EB含量与对照组相应时间点相比变化较大，平衡后小肠、脾、肺(Plt;0.05)及肿瘤(Plt;0.01)组织中EB含量比对照组升高，其他器官的改变无统计学差异。④组胺组各器官组织均未见明显水肿、出血表现，而瘤体内微血管明显扩张充血，新生血管中血浆成分渗出增加，瘤内及被膜下出血常见。结论 组胺能够增加EB在肿瘤局部的富集，适度组胺负荷不会给机体造成明显伤害。 【关键词】 组胺 黑色素瘤 血管壁 通透性 Abstract： Objective By investigating the effect of histamine on the microvessel permeability in melanoma B16 burdened C57BL/6 mice to provide an experimental rationale for the use of histamine in the antibody-coupled anti-tumor therapy. Methods Cultured melanoma B16 cells pit of C57BL/6 mice (200 μl each). Model mice l/kg through the caudal vein, folloine 300 mg/kg hypo. The control group e points of 10 min, 30 min, 1 h, 2 h and 4 h after injection, the mice ine the optic density of EB in internal organs and tumor tissue eter at 620 nm. The structural changes ined using histochemical stain. Results ① The formation of melanoma model in mice ice: redistribution ulation of EB , spleen and tumor; sloulation of EB, in the heart and kidneys; feine changed the distribution pattern of EB in the test group, causing apparent plasma level fluctuation. After equilibration, the EB level , spleen and lungs (Plt;0.05) and in the tumor (Plt;0.01), but not in the brain. ④ No apparent edema or hemorrhage could be seen in the internal organs in the histamine-treated model mice; but in the tumor，apparent microvessel congestion, increased plasma exudation, and bleeding or or under the pseudo-capsule ine increases the accumulation of EB in most tissues of the melanoma B16 burdened C57BL/6 mice, especially in the tumor tissue, causing no apparent injuries to the body, if histamine is not overloaded. Key ine; melanoma; vessel eability 肿瘤是危害人类健康的三大疾病之一。常规的术后放、化疗各有其局限性。