早期慢性肾脏疾病心脏病变的实验研究.pdfVIP

老年医学与保健2008年第14卷第1期 Geriatr Health Care，2008，V01．14．No 1 早期慢性肾脏疾病心脏病变的实验研究 杜国伟 鲍晓荣 【摘要】目的 研究早期慢性肾脏疾病大鼠心脏病变情况。方法 通过减少肾脏切除量和缩短喂养时间方法建 立早期慢性肾脏疾病大鼠动物模型，分析其心脏病变，包括心脏重，体重比值、心肌细胞增大、心肌间质纤维化程度 和心肌间质I型、Ⅲ型胶原含量等。结果 (1)实验组大鼠血尿素氮水平、尿白蛋白，肌酐比值、肾小球系膜增生程度 均高于对照组，但实验组和对照组之间血肌酐水平差异无统计学意义．且病理改变较轻，表明早期CKD模型制作是 成功的。(2)实验组大鼠心脏重，体重比值高于对照组 0．01)，心肌细胞肥大，心肌问质纤维化明显。免疫组化测定 显示心肌组织内I型和Ⅲ型胶原量均高于对照组(P均0．01 o结论 心脏病变在早期CKD大鼠动物模型中即已经存 在。表现为重量增加、间质纤维化、胶原增多等。 【关键词】慢性病； 肾疾病 心脏病； 心肌； 大鼠 ， 【中图分类号】R54 【文献标识码】A 【文章编号】1008—8296(2008)-01—0026一o4 Cardiac impairment in early chronic kidney disease：an experimental study in rats DU Guo—wei，BA0 Xiao— wng Department qfNephrology，Jinshan Hospital，Fudan University，Shanghai 200540，China 【Abstract】objective To examine cardiac impairment in early chronic kidney disease(CKD)in a rat mode1．Methods The early CKD animal model was established by subtotal nephreetomy(70％．4-2．0％)and shortening the feeding time of the rats(10 weeks)．Heart impairment was examined with respect to the heart／body weight ratio，hypertrophy of cardiomy0eytes and interstitial fibrosis．Collagen I and Ill in myocardial tissue were measured by immunohistochemistry Results (1)The meau percentage of removed kidney was 70．14-4-2．00％．In the experimental group，the main pathological change of the kidney was glomenflar mesangial proliferaticm．Focal segmental glomerular sc1erosis was rare．Blood urea nitrogen(BU N)，the urinmy albumin／creatinine ratio and the degl‘ee of glomendar mesangial proliferation were higher in the experimental group than those in the control group 0．01)，but there was no sigaaifieant difference in seHlm creatinine hetween the two groups；(2)Compared with the COntrol group，the heart，h0dy weight ratio was higher(eO．O1)and patholog