一种新型Zn_2_近红外荧光探针的研究.pdfVIP

2007 65 Vol. 65, 2007 13 , ACTA CHIMICA SINICA No. 13, · · Zn2 * ( 410082) Zn2, , Zn2+. TAEA-IR-780, Zn2, . , pH Zn2. 683 nm, 750 nm, , . Zn2 1.0 109 mol•L1, . ; ; Zn2 A Novel Near-infrared Fluorescent Probe for Zn2 TANG, You-Yun LUO, Chun-Hua WU, Zhao-Yang* SHEN, Guo-Li YU, Ru-Qin (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082) 2 Abstract Zn is an essential component for many enzymes and transcription factors, in which it plays structural or catalytic roles, so its detection is of great biological significance. The development of novel 2 near-infrared fluorescent probe for Zn , TAEA-IR-780, was described in this paper, which was obtained by directly attaching an acceptor, tris(2-aminoethyl)amine (TAEA), to a near-infrared fluorophore IR-780. The excitation and emission wavelengths of the TAEA-IR-780 are 683 and 750 nm, respectively, and its fluores- 2 cent intensity increases with the addition of Zn under physiological condition (pH 7.4) and the detection 9 1 limit reaches 1.0 10 mol•L . Moreover, the fluorescent intensity of TAEA-IR-780 does not increase no- 2 2 ticeably in the presence of other biologically important cations such as Ca and Mg . The results show that the probe can not only effectively minimize cell