cloning and expression studies of a lfy cdna from brassica juncea从芸苔属植物的克隆和表达研究lfy cdna juncea.pdfVIP
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cloning and expression studies of a lfy cdna from brassica juncea从芸苔属植物的克隆和表达研究lfy cdna juncea
Advances in Bioscience and Biotechnology, 2011, 2, 248-254 ABB
doi:10.4236/abb.2011.24036 Published Online August 2011 (http://www.SciRP.org/journal/abb/).
Cloning and expression studies of a LFY cDNA from Brassica
juncea
Suchandra Deb Roy, Mukesh Saxena, Neera Bhalla-Sarin
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
E-mail: neerasarin@
Received 1 March 2011; revised 9 April 2011; accepted 25 April 2011.
ABSTRACT (AP3) [2,8-10].
The expression of LFY gene was reported to increase
A LEAFY cDNA was cloned from the short-day (SD) with the age of the plant till it reached a threshold level
plant Brassica juncea cv Varuna. (BjLFY) consists of which is required for transition from vegetative to the
1261 bp encoding for a protein of 420 amino acids reproductive phase [11]. Overexpression of the LFY gene
and an estimated isoelectric point of 6.8. The deduced has been found to accelerate flowering in a variety of
amino acid showed 99% and 86% identity to Arabi- plants like, aspen and tobacco [12], rice [13], poplar [14]
and Citrus [15]. This ability to accelerate flowering has
dopsis thaliana and Brassica oleracea LFY cDNA re-
generated considerable interest in its role in transition
spectively. The LFY transcript was detected through-
from vegetative to the reproductive phase and its potential
out the ve
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