comparative analysis of fatty acid desaturases in cyanobacterial genomes蓝藻的脂肪酸desaturases基因组进行比较分析.pdfVIP

Hindawi Publishing Corporation Comparative and Functional Genomics Volume 2008, Article ID 284508, 25 pages doi:10.1155/2008/284508 Research Article Comparative Analysis of Fatty Acid Desaturases in Cyanobacterial Genomes Xiaoyuan Chi,1, 2, 3 Qingli Yang,1, 2, 3 Fangqing Zhao,1, 2 Song Qin,1 Yu Yang,1, 2 Junjun Shen,1, 2 and Hanzhi Lin1, 2 1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 Graduate University, Chinese Academy of Sciences, Beijing 100039, China 3 Shandong Peanut Research Institute, Qingdao 266100, China Correspondence should be addressed to Song Qin, sqin@ Received 23 August 2007; Revised 17 March 2008; Accepted 4 September 2008 Recommended by John Parkinson Fatty acid desaturases are enzymes that introduce double bonds into the hydrocarbon chains of fatty acids. The fatty acid desaturases from 37 cyanobacterial genomes were identified and classified based upon their conserved histidine-rich motifs and phylogenetic analysis, which help to determine the amounts and distributions of desaturases in cyanobacterial species. The filamentous or N2 -fixing cyanobacteria usually possess more types of fatty acid desaturases than that of unicellular species. The pathway of acyl-lipid desaturation for unicellular marine cyanobacteria Synechococcus and Prochlorococcus differs from that of other cyanobacteria, indicating different phylogenetic histories of the two genera from other cyanobacteria isolated from freshwater, soil, or symbiont. Strain Gloeobacter violaceus PCC 7421 was isolated from calcareous rock and lacks thylakoid membranes. The types and amounts of desaturases of this strain are distinct to those of other cyanobacteria, reflecting the earli