correlation between group b streptococcal genotypes, their antimicrobial resistance profiles, and virulence genes among pregnant women in lebanonb组链球菌基因型之间的相关性,它们的抗菌素耐药性资料、孕妇在黎巴嫩和毒力基因.pdfVIP

Hindawi Publishing Corporation International Journal of Microbiology Volume 2009, Article ID 796512, 6 pages doi:10.1155/2009/796512 Research Article Correlation between Group B Streptococcal Genotypes, Their Antimicrobial Resistance Profiles, and Virulence Genes among Pregnant Women in Lebanon Antoine Hannoun,1 Marwa Shehab,2 Marie-Therese Khairallah,2 Ahmad Sabra,2 Roland Abi-Rached,2 Tony Bazi,1 Khalid A. Yunis,3 George F. Araj,4 and Ghassan M. Matar2 1 Department of Obstetrics and Gynecology, Faculty of Medicine, American University of Beirut,Beirut, Lebanon 2 Department of Microbiology and Immunology, Faculty of Medicine, American University of Beirut,Beirut, Lebanon 3 Department of Pediatrics and Adolescent Medicine, Faculty of Medicine, American University of Beirut,Beirut, Lebanon 4 Department of Pathology and Laboratory Medicine, Faculty of Medicine, American University of Beirut,Beirut, Lebanon Correspondence should be addressed to Ghassan M. Matar, gmatar@.lb Received 6 July 2009; Accepted 30 November 2009 Recommended by Ingolf F. Nes The antimicrobial susceptibility profiles of 76 Streptococcus agalactiae (Group B Streptococci [GBS]) isolates from vaginal specimens of pregnant women near term were correlated to their genotypes generated by Random Amplified Polymorphic DNA analysis and their virulence factors encoding genes cylE, lmb, scpB, rib, and bca by PCR. Based on the distribution of the susceptibility patterns, six profiles were generated. RAPD analysis detected 7 clusters of genotypes. The cylE gene was present in 99% of the isolates, the lmb in 96%, scpB in 94.7%, rib in 33%, and bca in 56.5% of isolates. The isolates demonstrated a significant correlation between antimicrobial resistance and ge