烟草NtLS基因RNA干扰表达载体的构建及遗传转化-中国烟草科学.PDF

2011-08 ，32 （4 ） Chinese Tobacco Science 31 NtLS RNA * 266101 GRAS RACE NtLS GenBank EU935581NtLS LS/LAS/MOC1 RNAi W38NtLS NtLS RNA TS413 1007-5119201104-0031-05 DOI 10.3969/j.issn.1007-5119.2011.04.008 Construction of RNAi Vector of NtLS Gene and its Transformation in Tobacco * WANG Weifeng, TAI Shuaishuai, WANG Lu, LIU Guanshan, LI Fengxia, GAO Xiaoming, SUN Yuhe (Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China) Abstract: Axillary bud formation involves many regulatory genes, one of key factors belongs to the plant specific protein family GRAS. Mutation or function missing of these genes would affect the axillary formation. The tobacco NtLS gene (EU935581) was cloned by RACE technology in our lab. The sequences of NtLS are highly similar to those of LS/LAS/MOC1 , which regulate the formation of axillary meristems. The interference fragments were chosen from NtLS and the RNAi expression vectors were constructed and transformed into Nicotiana tabacum W38 by agrobacterium-mediated leaf disc transformation methods, to form basis for further study of NtLS function. Keywords: tobacco; NtLS ; RNAi GRAS [1] RACE NtLS GenBank EU935581cDNA 1 847 bp1 224 bp407 [2] [6]NtLS LS/LAS/MOC1 NtLS