成年态南丰蜜橘试管嫁接育苗技术研究-热带亚热带植物学报.PDF

热带亚热带植物学报　2014 ，22(2) ：179 ~ 184 Journal of Tropical and Subtropical Botany 成年态南丰蜜橘试管嫁接育苗技术研究 丁明华, 吉枝单, 涂艺声* (江西师范大学生命科学学院， 南昌 330022) 摘要： 为探索适合成年态南丰蜜橘[Citrus reticulata Blanco ‘kinokuni’ (Tanaka) H. H. Hu]的快速繁殖技术 ，对其试管茎尖微嫁 接育苗进行研究 。结果表明，最好 的砧木是苦柚种子苗 ，以腹接方式 的成活率最高。嫁接苗接种在 MS + GA3 1 mg L– 1 + 蔗糖 – 1 75 g L 的培养基 中，暗培养 7 d 后转入光周期下培养 ，嫁接成活率达 67.78%。不 同移栽基质对嫁接苗 的成活率影响不显著 。 嫁接苗与成年态南丰蜜橘再生芽在形态和 POD 、CAT 及 SOD 同工酶分子表达上均无 明显差异 。这表明通过试管茎尖微嫁接 技术可保持其遗传稳定性 。 关键词： 柑橘 ；微嫁接 ；成活率 ；同工酶 doi: 10.3969/j .issn.1005–3395.2014.02.011 ‘ ’ Studies on in vitro Micrografting of Mature Citrus reticulata Blanco Kinokuni (Tanaka) H. H. Hu * DING Ming-hua, JI Zhi-dan, TU Yi-sheng (College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China) Abstract: In order to exploe the rapid propagation of mature Citrus reticulata Blanco ‘Kinokuni’ (Tanaka) H. H. Hu, the breeding technology by in vitro micrografting was studied. The results showed that the most suitable rootstock was bitter pomelo [C. maxima (Burm.) Merr. ‘Maxima’] seedling and the side grafting method had the highest survival. The micrografting seedlings cultured in MS medium with 1 mg L–1 GA and 75 g L–1 sugar 3 were at first grown in dark for 7 days, then transferred to light with 10 h d–1 , which survival cultured after 25 days reached 67.78%. The transplanting substrate had not significant influence on survival of grafting seedlings. Furthermore, there were no obvious differences between grafting seedlings and adventitious buds in morphology, as well as the isozyme expression of POD, CAT and SOD. It was suggested that the in vitro micografting technique could keep the genetic stability of graf