干旱胁迫条件下马铃薯耐旱品种宁蒗182叶片蛋白质组学分析-遗传.PDF

HEREDITAS (Beijing) 2013 5 , 35(5): 666―672 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2013.00666 干旱胁迫条件下马铃薯耐旱品种宁蒗182 叶片蛋白质 组学分析 1,2,3 3 4 1,2 1,2 1,2 章玉婷 , 周德群 , 苏源 , 余萍 , 周晓罡 , 姚春馨 1. , 650223; 2. , 650223; 3. , 650093; 4. , 650214 开展马铃薯抗旱分子机理的研究对培育马铃薯抗旱品种, 减少干旱造成的损失至关重要。文章利用双向 电泳技术对云南地方耐旱马铃薯品种宁蒗182 在干旱与正常处理条件下叶片表达差异蛋白质组进行对比研究。 经电泳图谱分析和 MALDI-TOF-TOF/MS 质谱鉴定获得 12 个表达差异蛋白点, 并进行了功能分类。结果发现, 在差异蛋白中具有保护马铃薯光和系统以及线粒体正常运转的酶类; 调节该植株对环境胁迫响应的信号传导 以及调控其组织内N 、C 运输系统的功能蛋白, 这些蛋白在受到干旱胁迫时表达量均升高。这一结果揭示出该 类蛋白是马铃薯在干旱条件下产生的耐受相关蛋白。文章为阐释马铃薯抗旱品种通过多种路径和水平的调控提 高其抗性的分子机理提供了理论依据。 干旱胁迫; 双向电泳; 耐旱品种; 蛋白质组 Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress 1,2,3 3 4 1,2 1,2 ZHANG Yu-Ting , ZHOU De-Qun , SU Yuan ,YU Ping , ZHOU Xiao-Gang , YAO Chun-Xin 1,2 1. Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China; 2. Key Laboratory of Agricultural Biotechnology of Yunnan Province , Kunming 650223, China; 3. Environmental Science and Engineering Faculty, Kunming University of Science and Technology, Kunming 650093, China; 4. Life Science and Technology Faculty, Kunming University, Kunming 650214, China Abstract: It is utmost important to cultivate potato variety with drought resistance to reduce arid loss by research pro- gress on potato drought resistance molecular mechanism. A comparative study with differences in protein group analysis of potato drought resistance variety in Ninglang 182 leaves was carried out by