ACA,APA微囊的强度,通透性及生物相容性研究.docVIP

ACA、APA微囊的强度、通透性及生物相容性研究 刘锐 蔡善君* 詹文芳 谢兵 李红 (遵义医学院附属医院眼科，贵州遵义563003) 摘要： 目的 观察海藻酸钠-壳聚糖-海藻酸钠(alginate-chitosan-alginate, ACA) 微囊、海藻酸钠-多聚赖氨酸-海藻酸钠(alginate-polylysine-alginate, APA) 微囊的强度、通透性及生物相容性，评价ACA、APA 两种微囊在细胞移植中作为运载工具的可行性。方法　分别制备ACA微囊和APA微囊，采用37℃摇床振荡对这两种微囊机械强度进行比较研究。以胰蛋白酶、异硫氰酸荧光素标记的IgG、牛血清白蛋白BSA来观察囊膜的通透性。用ACA、APA 微囊包封293细胞，ACA 和APA 微囊囊膜的机械强度良好，所制备的APA微囊允许相对分子质量为22×103胰蛋白酶、相对分子质量为66×103牛血清白蛋白通过、两种微囊均限制相对分子质量为150×103大分子免疫球蛋白透过。回收移植10周ACA、APA 细胞微囊，其形态保持不变，回收微囊化的293细胞在体外进行再次培养，细胞形态正常，移植后大白兔眼组织未见明显炎症改变，大鼠的重要器官（心、肝、脾、肺、肾）无明显的病理改变。结论 ACA、APA微囊是细胞移植过程中包裹、保护细胞的合适载体,这为将来眼科临床应用奠定了基础。 【关键词】 海藻酸钠；壳聚糖；多聚赖氨酸；微囊；细胞移植；载体 Observation mechanical strength、permeability and　biocompatibility of ACA、APA microcapsule Liu Rui, CAI Shan-jun, ZHAN Wen-fang, Xie Bin，Li Hong, Department of Ophthalmology, the Affiliated Hospital of Zunyi Medical College, Zunyi，563003，China Corresponding author: Caishanjun，Email: caishanjun@163.com Abstract： Objective To investigate the mechanical strength、 permeability and biocompatibility of alginate-chitosan-alginate (ACA)、alginate-polylysine - alginate (APA) microcapsules and evaluate the feasibility of ACA、APA microcapsules as carriers of cell transplantation. Methods The alginate - chitosan - alginate microcapsules and the alginate - polylysine - alginate microcapsules were prepared separately. the mechanical strength of microcapsules was measured in the shaking apparatus at 37 ℃. The permeability of both microcapsules was observed with some proteins such as trypsin、immunoglobulin IgG and bovine serum albumin under fluorescent microscope .The ACA、APA microcapsules entrapping 293 cells were transplanted to the periorbit of New Zealand White rabbit and the abdominal cavity of mice .After microcapsules were retrieved termly, the viability of encapsulated cells and the biocompatibility of microcapsules were also examinated. Results Both the membranes of ACA and APA microcapsules were electrolyte complexes, having enough mechanic strength. APA memb