‘幻想’矮牵牛遗传转化受体再生体系建立.pdfVIP

安徽农业科学，Joumal of Anhui A伊. Sci.20日，41 (4) : 1462 -1463 , 1503 责任编辑朱琼琼责任校对卢荡 ‘幻想矮牵牛遗传转化受体再生体系建立 马方芳，岳远征，黄雪，孙健，胡惠蓉* (华中农业大学同艺林学学院，园艺植物生物学教育部重点实验室，湖北武汉 43∞70) 摘要 [目的]建立‘幻想矮牵牛的高效遗传转化受体再生体系。[方法]以‘幻想矮牵牛徽叶片为外植体，对其暗培养时间、出芽和生 根培养基的生长素种类及浓度、筛选抗生素和抑茵抗生素进行优化， [结果]诱导叶片分化的最佳暗培养时间为 2 d; 最佳出芽培养基为 MS +6-BA 2.0 mglL + NAA O. 2 mglL; 最佳生根培养基为 M5 + IBA O. 1 mglL; 适宜出芽和生根阶段的卡那霉素筛选压为 15 mglL; 适宜 的抑菌抗生素头抱霉素浓度为 3∞ mglLo [结论]该研究为后期‘幻想矮牵牛的分子及育种研究奠定了基础。 关键词 ‘幻想矮牵牛;遗传转化;再生体系;暗培养时间;生长亲;抗生素 中图分类号 568 1. 6 文献标识码 A 文章编号 0517 - 6611 (2013)04 -01462 -02 EstabIishment of Regeneration System for Genetic Transformation of Petunio: hybl也加‘ Fantasy MAF皿g.f:皿g et al (Key Laboratory of Horticultural Plant Biology , Ministηof Education , College of Horticulture and Forestry 5ciences , Huazhong Agricultural University , Wuhan , Hubei 43∞70) Abstract [ Objective] The paper was to establish efficient regeneration system for genetic transformation of Petunia hybrida ‘ Fantasy . [ Method]ηle effects of dark incubation time , auxin types and concentrations of sprouting medium and rooting medium , screening antibiotics and antibacteriál antibiotics on shoot regeneration and rooting were studied by using young leaves of P. hybrida ‘ Fantasy as explants. [Re- sult] 币le best dark incubation time for the induction of leaf differentiation was 2 days; the best medium for sprouting was MS + 6-BA 2.0 mglL + NAA O. 2 mglL; the best rooting medium was M5 + IBA O. 1 mglL; the appropriate kanamycin screening pressure in the sprouting and rooting stage w描 15 mglL; the suitable concentration of 朋an川t由1出ba削cterial an川巾t仙i池恤bilω川otics cef，岛otax刘ime was 3∞ mglL. [刊Conclusion叫] 四The 山stud句y laid the foundation for the late molecular and breeding research of P. hybrωα ， Fantasy . Key word凶 Petunia hybrida ‘ Fantasy ; Genetic transformation; Regeneration system; Oark inc