Livinα和Livinβ在儿童急性白血病骨髓单个核细胞中的表达及临床意义.docVIP

Livinα和Livinβ在儿童急性白血病骨髓单个核细胞中的表达及临床意义 毛成刚1，薛文婷2，徐酉华1，郭玥馨1 (1重庆医科大学附属儿童医院血液科，重庆400014) 摘 要： 目的 研究Livinα和Livinβ在儿童急性白血病（AL）骨髓中的表达与临床意义。用实时定量PCR（Real-Time Quantitative PCR，RQPCR）和esternblot检测Livinα和Livinβ儿童骨髓单个核细胞中mRNA和蛋白质的表达。 Livinα和Livinβ在初诊39例儿童急性淋巴细胞白血病（ALL）和12例急性髓白血病（AML）骨髓中的表达率和表达水平均高于对照组（P＜0.05），Livinα的表达水平高于Livinβ（P＜0.05）ALL化疗第19天，幼稚细胞≤5%组Livinα和Livinβ的表达水平化疗前幼稚细胞＞5%组P＜0.05），但化疗后Livinα和Livinβ的表达水平。 Livinα和Livinβ在儿童AL中的表达可能与儿童AL的发生和发展相关Livinα和Livinβ可作为观察儿童AL的分子指标之一。 Livin；；白血病Expression and clinical significance of Livinα and Livinβ in marrow Mononuclear cells of childhood acute leukemia MAO Cheng-gang1，XU You-hua1，GUO Yue-xin1 (1Department of Hematology，Children’s Hospital，Chongqing Medical University，Chongqing 400014Medicine，Second People，s Hospital of Qingdao Economic and Technological Development Zone，Qingdao 266500，China) Abstract： Objective To investigate the expression of Livinα and Livinβ in marrow mononuclear cells （MMNC）of Childhood Acute Leukemia(CAL) and explore their clinical significance. Methods Real-Time Quantitative PCR and Western blot were used tothe expression of mRNA and protein of Livinα and Livinβ in MMNC of CAL，respectively. Results Both Livinα and Livinβ expression rates and expression levels were higher in preliminary diagnosis group（n=51）of CAL ，including acute lymphoblastic leukemia（n=39）and acute myeloid leukemia （n=12), compared with the control group（P＜0.05）, and the expression level of Livinα was higher than Livinβ（P＜0.05）. The expression levels of Livinα and Livinβ were significantly lower in patients with a favorable day 19 bone marrow response (leukemic blasts ≤5%) to induction chemotherapy of ALL than in the patients without chemotherapy（P＜0.01）. Though the expression levels of Livinα and Livinβ were also lower in patients with a unfavorable day 19 bone marrow response (leukemic blasts＞5%) to induction chemotherapy of ALL than in the patients without chemotherapy（P＜0.05）, the expression levels of Livinα and Livinβ were