不同感染途径建立的结核分枝杆菌急性肺感染小鼠模型的比较.pdfVIP

• 76 • 中华传染病杂志 2012 年 2 月第 30 卷第 2 期 Chin J Infect Dis , February 2012 ,Vo l. 30 , No.2 ·基础论著· 不同感染途径建立的结核分枝杆菌 急性肺感染小鼠模型的比较 刘芳杨华周文江范小勇王德、成 E摘要】 目的 比较 3 种不同感染途径建立的结核分校杆菌急性肺感染小鼠动物模型，为结 核病研究中动物模型的建立、选择与实际应用提供实验依据。方法 将结核分校杆菌标准株 H37Rv 稀释至 1 X 10 菌落形成单位(cfu)/mL ，分别采用尾静脉注射、滴鼻、气雾攻击方式感染小 鼠，感染后 6 周，观察肺组织病变情况、菌落计数，肺组织行 HE 染色、抗酸染色，免疫组织化学检测 肺组织 TNFα 单位面积表达量。数据行t 检验。结果 气雾攻击组、滴鼻组和尾静脉组小鼠肺部结 核分校杆菌数分别为(6.290士0:028) 、 (6. 150 士0.02 1)和 (6.120 ::1:: 0.008) 19 cfu/mL ，对照组肺部无 结核分枝杆菌;各感染组与对照组比较，差异均有统计学意义(t=3. 762 , PO.01)， 但不同感染途 径感染组间差异无统计学意义(PO.O日。各感染组小鼠肺组织均有病理学改变，抗酸染色阳性。 单位面积 TNF-α 表达量在尾静脉注射组、滴鼻组和气雾攻击组分别为 O. 049 X 10 、 0.759 X 10 和 1. 042X 10 ，差异有统计学意义(t=2. 504 ,P0. 05) 。结论 气雾攻击感染方式建立的小鼠急性肺 结核病模型较尾静脉注射和滴鼻方式更为有效。 E关键词】 注射，静脉内p 投药，鼻内;气雾剂z 结核，肺;疾病模型，动物 Comparison of acute Mycobacterium tuberculosis infection mouse models established by different infection routes LIU Fang 势 ， YANG Hua , ZHOUWen-jiang , FAN Xia伊yong ,WANG De-cheng. • Center of Animal , Shanghai Public H ealth Clinical Center , Shanghai 201508 , China Corresρondingauthor: ZHOUWen-jiang , Email:wjzhou@shmu.cn [Abstractl Objective To compare three types of acute Mycobacterium tuberculosis infection mouse models established through different infection routes and to set up the theoretical basis for further developing , selecting and applying these animal model in the tuberculosis-related research. Methods Standard strain of Tubercle bacillus H37Rv was diluted to 1 X 10 colony forming unit (cfu)/mL. The mice were infected with the bacteria through different routes including intravenous injection , intranasal administration and inhalation of bacteria aerosol. Six weeks after the infection , the mice were euthanized and necropsied. The lung tissues were collected and gross changes were observed. The colony counting was per