青海盐湖地区嗜盐菌的分离纯化及抑制植物病原菌的-广东农业科学.PDF

广东农业科学 2013 年第 1 期 79 青海盐湖地区嗜盐菌的分离纯化及 抑制植物病原菌的活性初探 沈 硕袁 王 舰 青海省农林科学院生物技术研究所/青藏高原生物技术教育部重点实验室袁青海西宁 810016冤 摘要 以番茄灰霉菌 Botrytis c inerea冤尧菊芋菌核菌 Jerusalem artichoke Sclerotium冤尧油菜菌核菌 Sclerotinia sc le rotiorum冤尧辣 椒疫霉菌 Phytophthora capsici冤尧蚕豆根腐菌 Fusarium solani冤及豌豆根腐菌 A phanomyces euteiches Dreehsler 冤等 6 种植物病原真 菌为供试菌株袁采用滤纸片法对植物病原菌提取液的抑菌活 进行了测定遥 为了获得对这几种植物病原菌具有较高抑制作用的 嗜盐菌菌株袁对供试菌株进行了液体发酵及发酵液的活 筛选遥 经滤纸片法测定袁青海盐湖嗜盐菌菌株对辣椒疫霉菌具有较强的 拮抗作用袁并用最小抑制浓度法 MIC冤测定了嗜盐菌提取物对辣椒疫霉菌的最小抑菌浓度范围为 9~12 mg/mL遥 关键词 嗜盐菌曰 次级代谢产物曰 滤纸片法曰 抑菌活 垣 中图分类号 杂源愿圆援圆 怨圆 文献标识码 A 文章编号 1004-874X 2013冤01-0079-03 Isolation, petrifaction and inhibitory activity against plant pathogenic fungi of halophilic strains from Qinghai Salt Lake SHEN Shuo, WANG Jian (Institute of Biotechnology of Qinghai A cade my of A griculture and Forestry /The Tibet Plateau Biotec hnology Key Lab of Ministry of Education, X ining 810016, China) Abstract: Six plant pathogenic fungi including Botrytis cine rea, Jerusale m artichoke Sclerotium, Sclerotinia sclerotiorum, Phytophthora capsici, Fusarium solani, and A phanomyces euteiches Dreehsler were used to determine the inhibitory activity of fermentation broth of halophilic bacteria against plant pathogenic fungi by paper disc diffusion method. In order to find halophilic strain with higher inhibitory activity against the plant pathogenic fungi, the tested strains were fermented to screen. The halophilic stra