analysis of kras mutations of exon 2 codons 12 and 13 by snapshot analysis in comparison to common dna sequencing分析kras突变的外显子2基码12和13个快照相比常见的dna测序分析.pdfVIP

Hindawi Publishing Corporation Gastroenterology Research and Practice Volume 2010, Article ID 789363, 5 pages doi:10.1155/2010/789363 Research Article Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing 1 ¨ ¨ 2 1 2 Rica Zinsky, Servet Bolukbas, Holger Bartsch, Joachim Schirren, and Annette Fisseler-Eckhoff1 1 Institute of Pathology and Cytology, HSK Wiesbaden, Academic Hospital of University of Mainz, Ludwig-Erhard-Strasse 100, 65199 Wiesbaden, Germany 2 Department of Thoracic Surgery, HSK Wiesbaden, Academic Hospital of University of Mainz, Ludwig-Erhard-Strasse 100, 65199 Wiesbaden, Germany Correspondence should be addressed to Rica Zinsky, zinsky@pathologie-wiesbaden.de Received 24 September 2010; Revised 10 November 2010; Accepted 10 November 2010 Academic Editor: Antoni Castells Copyright © 2010 Rica Zinsky et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Due to the call for fast KRAS mutation status analysis for treatment of patients with monoclonal antibodies for metastatic colorectal cancer, sensitive, economic, and easily feasible methods are required. Under this aspect, the sensitivity and specificity of the SNaPshot analysis in comparison to the commonly used DNA sequencing was checked. We examined KRAS mutations in exon 2 codons 12 and 13 with DNA sequencing and SNaPshot analysis in 100 formalin-fixed paraffin-embedded tumor tissue samples of pancreatic carcinoma, colorectal carcinoma, and