assessment of a standardized ros production profile in humans by electron paramagnetic resonance评估一个标准化的电子顺磁共振ros在人类生产状况.pdfVIP

Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2012, Article ID 973927, 10 pages doi:10.1155/2012/973927 Research Article Assessment of a Standardized ROS Production Profile in Humans by Electron Paramagnetic Resonance Simona Mrakic-Sposta,1 Maristella Gussoni,1 Michela Montorsi,2, 3 Simone Porcelli,2, 3 and Alessandra Vezzoli3 1 Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti, Universita di Milano, Via Fratelli Cervi 93, 20090 Segrate, Italy ` 2 Universita Telematica S. Raff aele Roma, Via F. Daverio 7, 20122 Milan, Italy ` 3 Istituto di Bioimmagini e di Fisiologia Molecolare, Consiglio Nazionale delle Ricerche, Via Fratelli Cervi 93, 20090 Segrate, Italy Correspondence should be addressed to Alessandra Vezzoli, alessandra.vezzoli@r.it Received 14 March 2012; Revised 6 June 2012; Accepted 7 June 2012 Academic Editor: Steve R. McAnulty Copyright © 2012 Simona Mrakic-Sposta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Despite the growing interest in the role of reactive oxygen species (ROS) in health and disease, reliable quantitative noninvasive methods for the assessment of oxidative stress in humans are still lacking. EPR technique, coupled to a specific spin probe (CMH: 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine) is here presented as the method of choice to gain a direct measure- ment of ROS in biological fluids and tissues. The study aimed at demonstrating that, differently from currently available “a poste- riori” as