cd9 expression by human granulosa cells and platelets as a predictor of fertilization success during ivfcd9的表达由人类颗粒细胞和血小板在试管婴儿受精成功的预测.pdfVIP

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cd9 expression by human granulosa cells and platelets as a predictor of fertilization success during ivfcd9的表达由人类颗粒细胞和血小板在试管婴儿受精成功的预测.pdf

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cd9 expression by human granulosa cells and platelets as a predictor of fertilization success during ivfcd9的表达由人类颗粒细胞和血小板在试管婴儿受精成功的预测

Hindawi Publishing Corporation Obstetrics and Gynecology International Volume 2010, Article ID 192461, 7 pages doi:10.1155/2010/192461 Research Article CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF Carolyn R. Jaslow,1 Kyle S. Patterson,2 Shila Cholera,3 Lisa K. Jennings,3 Raymond W. Ke,2, 4 and William H. Kutteh2, 4 1 Department of Biology, Rhodes College, 2000 North Parkway, Memphis, TN 38112, USA 2 Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN 38152, USA 3 Department of Internal Medicine, The Vascular Biology Center of Excellence, University of Tennessee Health Science Center, Memphis, TN 38152, USA 4 Fertility Associates of Memphis, 80 Humphreys Center, Suite 307, Memphis, TN 38120, USA Correspondence should be addressed to William H. Kutteh, wkutteh@ Received 27 May 2010; Revised 16 July 2010; Accepted 28 July 2010 Academic Editor: Edward V. Younglai Copyright © 2010 Carolyn R. Jaslow et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Objective. To determine whether CD9 expression on human granulosa cells (GCs) and platelets could predict the success of conventional fertilization of human oocytes during in vitro fertilization (IVF). Methods. Thirty women undergoing IVF for nonmale factor infertility participated. Platelets from venous blood and GCs separated from retrieved oocytes were prepared for immunoﬂuorescence. Flow cytometry quantiﬁed the percent of GCs expressing CD9, and CD9 surface density on GCs and platelet