role of mn2+ and compatible solutes in the radiation resistance of thermophilic bacteria and archaeamn2 +和兼容的溶质在嗜热细菌和古菌的辐射抗性.pdf

Hindawi Publishing Corporation Archaea Volume 2012, Article ID 845756, 11 pages doi:10.1155/2012/845756 Research Article Role of Mn2+ and Compatible Solutes in the Radiation Resistance of Thermophilic Bacteria and Archaea Kimberly M. Webb and Jocelyne DiRuggiero Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA Correspondence should be addressed to Jocelyne DiRuggiero, jdiruggiero@ Received 25 July 2012; Revised 18 September 2012; Accepted 13 October 2012 Academic Editor: Yoshizumi Ishino Copyright © 2012 K. M. Webb and J. DiRuggiero. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Radiation-resistant bacteria have garnered a great deal of attention from scientists seeking to expose the mechanisms underlying their incredible survival abilities. Recent analyses showed that the resistance to ionizing radiation (IR) in the archaeon Halobacterium salinarum is dependent upon Mn-antioxidant complexes responsible for the scavenging of reactive oxygen species (ROS) generated by radiation. Here we examined the role of the compatible solutes trehalose, mannosylglycerate, and di-myo- inositol phosphate in the radiation resistance of aerobic and anaerobic thermophiles. We found that the IR resistance of the thermophilic bacteria Rubrobacter xylanophilus and Rubrobacter radiotolerans was highly correlated to the accumulation of high intracellular concentration of trehalose in association with Mn, supporting the model of Mn2+ -dependent ROS scavenging in the aerobes. In contrast, the hyperthermophilic archaea Thermococcus gammatolerans and Pyroc