fluorescence anisotropy of oligomeric proteins寡聚蛋白质的荧光各向异性.pdfVIP

Spectroscopy 18 (2004) 85–93 85 IOS Press Fluorescence anisotropy of oligomeric proteins Daniel M. Czajkowsky Department of Molecular Physiology and Biological Physics and Biophysics Program, University of Virginia School of Medicine, Charlottesville, VA 22908, USA Tel.: +1 434 924 1231; Fax: +1 434 982 1616; E-mail: dmc2m@ Abstract. Previous studies of protein oligomerization using time-resolved fluorescence anisotropy assumed a single fixed probe per oligomeric complex and an identical