柴胡皂甙d对人肝癌细胞STAT3COX-2信号通路调节作用.docVIP

柴胡皂甙d对人肝癌细胞STAT3/COX-2信号通路调节作用 朱占芳1和水祥1侯和磊1卢新兰11 , 陈静宏2 王治沦2(710061陕西 西安西安交通大学第一附属医院消化内科1710061陕西 西安西安交通大学医学院地方性骨病研究所2) 摘要体外培养人肝癌SMMC-7721细胞，经IL-6、AG490作用后，免疫细胞化学及免疫印迹方法检测STAT3、磷酸化STAT3(p-STAT3)以及COX-2蛋白表达的变化。IL-6诱导组p-STAT3和COX-2蛋白的表达较空白对照组显著升高，AG490或SSd后p-STAT3、COX-2蛋白表达较IL-6诱导组明显对STAT3蛋白表达IL-6作用人肝癌细胞p-STAT3和COX-2表达AG490或SSd作用的人肝癌细胞p-STAT3表达呈现剂量依赖性下降，COX-2蛋白表达相应下调。p-STAT3参与了人肝癌细胞COX-2表达d可通过抑制下调COX-2的表达抗肿瘤。关键词：肝癌环氧化酶-2信号传导与激活子3柴胡皂甙d中图分类号：R735.7 文献标码: A 文章编号：Effects of Saikosaponin d on STAT3/COX-2 Signal Transduction Pathway in Hepatocellular Carcinoma Cell Zhu Zhanfang1, He Shuixiang1, Hou Helei 1, Lu XinLan 1, Zhai Xuemin1, Chen Jinghong2, Wang Zhilun2 （1Department of Gastroenterology，The First Affiliated Hospital，College of Medicine；2. Department of Public Health，College of Medicine Xi’an Jiaotong University，Xi’an 710061，Shan Xi，China) [Abstract] Objective To observe influence of aikosaponin-d on the expression of STAT3 and COX-2 in hepatocellular carcinoma cells, and explore the underlying signal pathway mechanism of anti-tumor by saikosaponins-d. Methods The human hepatocell- ular carcinoma SMMC-7721 cells cultured in vitro was treated with IL-6, AG490 and saikosaponins-d, the protein expression of STAT3, p-STAT3 and COX-2 were measured by immunocytochemistry as well as western blotting methods, explore their relationship furemore. Results Immunocytochemistry showed that compared with blank group, both p-STAT3 and COX-2 were obviously increased in SMMC-7721 cells exposed to IL-6. Compared with IL-6 group, the protein level of COX-2 was decreased by AG490 or saikosaponins-d accompany with the expression of p-STAT3. While the expression of stat3 have no difference in any groups. The western blotting further confirmed the upper results. Conclusion: p-STAT3 is involved the expression of COX-2 in SMMC-7721 cells. Saikosaponin-d can down-regulate the expression of COX-2 partially working through inhibiting STAT3 phosphorylation. [keywords] Hepatocellular