a novel gene signature for molecular diagnosis of human prostate cancer by rt-qpcr一种新型基因签名供rt-qpcr人类前列腺癌的分子诊断.pdfVIP

A Novel Gene Signature for Molecular Diagnosis of Human Prostate Cancer by RT-qPCR 1,2. 1,2. 3 3 4 Federica Rizzi , Lucia Belloni , Pellegrino Crafa , Mirca Lazzaretti , Daniel Remondini , Stefania 5 5 6 1,2 Ferretti , Piero Cortellini , Arnaldo Corti , Saverio Bettuzzi * 1 Department of Medicina Sperimentale, University of Parma, Parma, Italy, 2 Istituto Nazionale Biostrutture e Biosistemi (I.N.B.B.), Roma, Italy, 3 Department of Patologia e Medicina di laboratorio, University of Parma, Parma, Italy, 4 Dimorfipa, University of Bologna, Bologna, Italy, 5 Urology Operative Unit, Azienda Ospedaliera-Universitaria of Parma, Parma, Italy, 6 Department of Scienze Biomediche,University of Modena, Modena, Italy Abstract Background: Prostate cancer (CaP) is one of the most relevant causes of cancer death in Western Countries. Although detection of CaP at early curable stage is highly desirable, actual screening methods present limitations and new molecular approaches are needed. Gene expression analysis increases our knowledge about the biology of CaP and may render novel molecular tools, but the identification of accurate biomarkers for reliable molecular diagnosis is a real challenge. We describe here the diagnostic power of a novel 8-genes signature: ornithine decarboxylase (ODC), ornithine decarboxylase antizyme (OAZ), adenosylmethionine decarboxylase (AdoMetDC), spermidine/spermine N(1)-acetyltransferase (SSAT), histone H3 (H3), growth arrest specific gene (GAS1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Clusterin (CLU) in tumour detection/classification of human CaP. Methodology/Pr