原创力文档a novel immunological assay for hepcidin quantification in human serum一种新型免疫分析在人类血清hepcidin量化.pdfVIP
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a novel immunological assay for hepcidin quantification in human serum一种新型免疫分析在人类血清hepcidin量化
A Novel Immunological Assay for Hepcidin
Quantification in Human Serum
1 1 2 3
Vasiliki Koliaraki , Martha Marinou , Theodoros P. Vassilakopoulos , Eustathios Vavourakis , Emmanuel
4 2 4 3
Tsochatzis , Gerassimos A. Pangalis , George Papatheodoridis , Alexandra Stamoulakatou , Dorine W.
5 2 1
Swinkels , George Papanikolaou , Avgi Mamalaki *
1 Laboratory of Molecular Biology and Immunobiotechnology, Department of Biochemistry, Hellenic Pasteur Institute, Athens, Greece, 2 1st Department of Internal
Medicine and Department of Hematology, Athens University Medical School, Athens, Greece, 3 Hematology Laboratory, Aghia Sophia Children’s Hospital, Athens, Greece,
4 2nd Department of Internal Medicine, Athens University Medical School, Hippokration General Hospital of Athens, Athens, Greece, 5 Department of Clinical Chemistry,
Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands
Abstract
Background: Hepcidin is a 25-aminoacid cysteine-rich iron regulating peptide. Increased hepcidin concentrations lead to
iron sequestration in macrophages, contributing to the pathogenesis of anaemia of chronic disease whereas decreased
hepcidin is observed in iron deficiency and primary iron overload diseases such as hereditary hemochromatosis. Hepcidin
quantification in human blood or urine may provide further insights for the pathogenesis of disorders of iron homeostasis
and might prove a valuable tool for clinicians for the differential diagnosis of anaemia. This study describes a specific and
non-operator demanding immuno
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