醉香含笑根皮挥发性成分的GC-MS分析及对NIH-中国现代应用药学.PDF

·药物分析与检验· 醉香含笑根皮挥发性成分的GC-MS 分析及对NIH 3T3 细胞生长抑制 作用 宋晓凯(淮海工学院化学工程学院，江苏 连云港 222005) 摘要：目的 分析醉香含笑根皮的挥发性成分，并考察醉香含笑根皮提取物(REMMD)对小鼠成纤维细胞NIH 3T3 的体外 生长抑制作用。方法 水蒸气蒸馏法提取醉香含笑根皮的挥发性成分，用气相-质谱联用(GC-MS)技术分析，并用峰面积 归一化法测定各成分相对含量；采用MTT 法检测REMMD 对NIH 3T3 细胞体外生长抑制作用。结果 共检出57 个色谱 峰，确定了其中 31 种化合物，占该挥发性成分总量的 63.31%。结论 REMMD 的挥发性成分以 N ,N ’-二苯甲酰基-庚二 胺为主(28.97%) ，其他成分有棕榈酸及棕榈酸乙酯(7.03%) 、高香草酸(3.25%) 、4-[(1E)-3-羟基-1- 丙烯基]-2- 甲氧基-苯酚 (3.16%)、木香烯内酯(2.33%)、糠醛及其衍生物(2.5%)。25 ，50 ，75 ，100 μg·mL−1 的REMMD 能显着抑制NIH 3T3 细胞 的生长，其抑制率呈浓度依赖关系，提示其具有抗纤维化疾病的良好研究开发前景。 关键词：醉香含笑根皮；挥发性成分；GC-MS 分析；NIH 3T3 细胞；生长抑制 中图分类号：R917.101 ；R285.5 文献标志码：B 文章编号：1007-7693(2011)12-1122-04 GC-MS Analysis of Volatile Components from Roots of Michelia Macclurei Dandy. and Growth Inhibition of NIH 3T3 Cell Line SONG Xiaokai(College of Chemical Technology, HuaiHai Institute of Technology, Lianyungang 222005, China) ABSTRACT: OBJECTIVE The volatile components from roots of Michelia macclurei Dandy. were analysed, and the inhibition effects of root extract of Michelia macclurei Dandy. (REMMD) on growth of NIH 3T3 cell lines in vitro were explored. METHODS The volatile components from root of Michelia macclurei Dandy. were extracted with steam distillation method and analysed by GC-MS. The relative content of each component was calculated by peak area normalization. The inhibiting effects of REMMD on growth of NIH 3T3 cell lines in vitro were examined by MTT assay. RESULTS Fifty seven peaks were detected, in which 31 compounds were identified, which made up 63.31% of the total volatile components. CONCLUSION The main component in the volatile components is N , N ’-di-benzoyloxy-heptanediamide(28.97%), other components are palmitic acid and palmitic acid ethyl ester(7.03%), 4-hydroxy-3-methoxy phenyl acetic acid (3.25%), 4-[(1E)-3- hydroxy-1-propenyl]-2- methoxyphenol(3.16%), costunoli