慢性乙型肝炎病毒感染者血清TNF-α及IFN-γ水平分析.docVIP

慢性乙型肝炎病毒感染者血清TNF-α及IFN-γ水平分析[摘要] 目的：分析不同临床阶段的慢性HBV感染者血清TNF-α和IFN-γ水平差异，探讨其水平差异与慢性HBV感染结局的关系。方法：选择慢性乙型肝炎（CHB）87例，乙型肝炎肝硬化（LC）38例，HBV相关肝细胞癌（HCC）34例，应用ELISA法分别检测其血清IFN-γ和TNF-α水平。结果：在所观察的3组中，TNF-α水平均有明显升高，其中LC组和HCC组均显著高于CHB组（P＜0.001），但LC组和HCC组间无显著性差异；而CHB、LC和HCC 3组间血清IFN-γ水平无显著性差异（P=0.208）。结论：血清TNF-α水平可能与慢性HBV感染的结局相关，而IFN-γ水平与慢性HBV感染结局无明显相关性。 [关键词] 慢性HBV感染；IFN-γ；TNF-α [中图分类号] R512.6+2[文献标识码] B[文章编号] 1673-7210（2010）10（c）-069-02 Analysis of serum levels of TFN-α and IFN-γ in patients with Chronic Hepatitis B Virus Infection HOU Qingshun, ZHANG Haifeng, YAN Xiuping, LIU Fuhui, LI Jianzhong (Qingdao Infectious Disease Hospital, Qingdao 266033, China) [Abstract] Objective: To analyze the differences of serum levels of TNF-α and IFN-γ in different clinical stages of chronic hepatitis B virus infection, and explore the relationship between the levels and the outcomes of chronic HBV infection. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of TFN-α and IFN-γ in the serum of 87 patients with chronic hepatitis B (CHB), 38 patients of liver cirrhosis (LC), 34 patients of HBV related hepatocellular carcinoma (HCC). Results: In the observed 3 groups, the level of TNF-α was higher markedly in patients of LC group and HCC group than those in CHB group (P＜0.001), but there was no significant difference between LC group and HCC group; While there was no significant difference in the level of IFN-γ among 3 groups(P=0.208). Conclusion: Serum level of TNF-α may be correlated to the outcome of chronic HBV infection, while level of IFN-γ has no significant relevance. [Key words] Chronic hepatitis B virus infection; Interferon gamma; Tumor necrosis factors alpha 人体感染乙型肝炎病毒（hepatitis B virus, HBV）后主要是由细胞毒T细胞参与的细胞免疫应答进行清除，同时因细胞免疫应答导致肝损伤，而IFN-γ和TNF-α是人体感染HBV后重要的免疫应答细胞因子，其水平差异可能影响慢性HBV感染的结局。为了解血清IFN-γ和TNF-α水平在慢性HBV感染中的作用，本文对其进行了检测，并探讨其水平差异与慢性HBV感染结局的关系。 1 对象与方法 1.1 研究对象 选择我院住院的159例慢性HBV感染患者，包括慢性乙型肝炎（CHB）患者87例，乙型肝炎肝硬化（LC）患者38例，HBV相