气相色谱填充柱的制备（Preparation of gas chromatographic packed column）.docVIP

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气相色谱填充柱的制备（Preparation of gas chromatographic packed column）.doc

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气相色谱填充柱的制备（Preparation of gas chromatographic packed column）

气相色谱填充柱的制备（Preparation of gas chromatographic packed column） Gas chromatography, which has been used for most of the past 20 years, mostly uses a packed column and basically makes a filling of its own, so it is only a brief statement of the preparation of packed columns. Preparation of gas chromatographic packed column Gas chromatography is generally divided into packed column and capillary column, packed column for routine quantitative analysis of samples, especially high purity samples; capillary column separation for composition of complex or trace samples and residue analysis etc.. For the time being, the filling column is often used in the quantitative analysis of conventional and high-purity samples. Capillary column is generally commodity column, buy, and then instrument, aging can be used directly. Packed columns are mostly prepared by themselves, and column effects of packed columns are closely related to column preparation, especially for the preparation of fillers. Here is an example of the preparation of 5%OV-101/Chromosorb W HP glass chromatography column, simply talking about the preparation of packed column. Preparation of chromatographic column 1. Apply the fixing liquid: Name 0.5g OV-101 in 200mL beaker, add proper amount of chloroform to dissolve, dry 10 g The Chromosorb W HP carrier is gradually poured in so that the carrier is completely submerged. The beaker in a ventilated cabinet, in infrared drying, solvent evaporation and keep stirring until dry, then moved to 2H 110 C drying oven, spare. 2. Filling of chromatographic column: A small funnel is attached to the outlet of the dry chromatographic column, and the prepared filling is divided into the column at a time, while tapping the column wall continuously until it is filled to the outlet 1.5cm. Move the glass funnel to the entrance of the chromatographic column, At the exit end plug a small group by silylated glass wool and cotton by thin layer or 2 layers of gauze, through a rubber tube to v