antagonistic gene activities determine the formation of pattern elements along the mediolateral axis of the arabidopsis fruit拮抗基因活动决定的形成模式元素沿着中侧的轴的拟南芥水果.pdfVIP

Antagonistic Gene Activities Determine the Formation of Pattern Elements along the Mediolateral Axis of the Arabidopsis Fruit ´ 1 ´ 2 1 2 ´ Santiago Gonzalez-Reig , Juan Jose Ripoll , Antonio Vera , Martin F. Yanofsky , Antonio Martınez- Laborda1* ´ ´ ´ 1 Division de Genetica, Universidad Miguel Hernandez, San Juan de Alicante, Spain, 2 Division of Biological Sciences, University of California San Diego, La Jolla, California, United States of America Abstract The Arabidopsis fruit mainly consists of a mature ovary that shows three well defined territories that are pattern elements along the mediolateral axis: the replum, located at the medial plane of the flower, and the valve and the valve margin, both of lateral nature. JAG/FIL activity, which includes the combined functions of JAGGED (JAG), FILAMENTOUS FLOWER (FIL), and YABBY3 (YAB3), contributes to the formation of the two lateral pattern elements, whereas the cooperating genes BREVIPEDICELLUS (BP) and REPLUMLESS (RPL) promote replum development. A recent model to explain pattern formation along the mediolateral axis hypothesizes that JAG/FIL activity and BP/RPL function as antagonistic lateral and medial factors, respectively, which tend to repress each other. In this work, we demonstrate the existence of mutual exclusion mechanisms between both kinds of factors, and how this determines the formation and size of the three territories. Medial factors autonomously constrain lateral factors so that they only express outside the replum, and lateral factors negatively regulate the