antibody vh repertoire differences between resolving and chronically evolving hepatitis c virus infections抗体vh曲目解决和长期发展的丙型肝炎病毒感染之间的差异.pdfVIP

Antibody Vh Repertoire Differences between Resolving and Chronically Evolving Hepatitis C Virus Infections 1 1 2 2 2 1 Vito Racanelli *, Claudia Brunetti , Valli De Re , Laura Caggiari , Mariangela De Zorzi , Patrizia Leone , 1 1 1 Federico Perosa , Angelo Vacca , Franco Dammacco 1 Department of Internal Medicine and Clinical Oncology, University of Bari Medical School, Bari, Italy, 2 Experimental and Clinical Pharmacology Unit, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, Aviano (PN), Italy Abstract Despite the production of neutralizing antibodies to hepatitis C virus (HCV), many patients fail to clear the virus and instead develop chronic infection and long-term complications. To understand how HCV infection perturbs the antibody repertoire and to identify molecular features of antibody genes associated with either viral clearance or chronic infection, we ¨ sequenced the V(D)J region of naıve and memory B cells of 6 persons who spontaneously resolved an HCV infection (SR), 9 ¨ patients with a newly diagnosed chronically evolving infection (CE), and 7 healthy donors. In both naıve and memory B cells, the frequency of use of particular antibody gene subfamilies and segments varied among the three clinical groups, ¨ especially between SR and CE. Compared t