association of the gene polymorphisms ifn-γ +874, il-13 ？1055 and il-4 ？590 with patterns of reinfection with schistosoma mansoni协会的基因多态性ifn-γ+ 874,il-13 1055和il - 4 590模式的再感染曼氏裂体吸虫.pdfVIP

Association of the Gene Polymorphisms IFN-c +874, IL-13 21055 and IL-4 2590 with Patterns of Reinfection with Schistosoma mansoni 1 1 2 3 2 Michael R. Gatlin , Carla L. Black , Pauline N. Mwinzi , W. Evan Secor , Diana M. Karanja , Daniel G. Colley1* 1 University of Georgia, Athens, Georgia, United States of America, 2 Kenya Medical Research Institute, Kisumu, Kenya, 3 Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America Abstract Background: The immunologic findings that most consistently correlate with resistance in human schistosomiasis are high levels of IgE and low levels of IgG4. We have genotyped gene and promoter polymorphisms of cytokines associated with regulation of these isotypes in a cohort of men occupationally exposed to Schistosoma mansoni in western Kenya and evaluated their patterns with respect to resistance and susceptibility to reinfection after treatment and cure with praziquantel (PZQ). Methodology/Principal Findings: In this cohort, polymorphisms in IL-4 (2590T high IgE), IL-13 (21055T high producer) and IFN-c (+874A high producer) demonstrated several correlations with resistance to reinfection. Resistance to reinfection was significantly correlated with the heterozygous IL-4 2590 genotype C/T (OR 3.5, [CI 1.2, 10.2]) compared to T/T. Among men with a homozygous IL-13 genotype CC/TT, having a T allele at the IFN-c +874 position increased the odds of resistance relative to individuals with the IFN-c +874 A/A genotype (OR = 17.5 [CI 3.0, 101.5]). Among men with homozygous A/A IFN-c genotype, the heterozygous IL-13 genotype C/T was associated with resistance relative to the homozygous C/C or T/T genotypes (OR = 22.5