astrocytic mechanisms explaining neural-activity-induced shrinkage of extraneuronal space星形机制解释neural-activity-induced extraneuronal空间的收缩.pdfVIP

Astrocytic Mechanisms Explaining Neural-Activity- Induced Shrinkage of Extraneuronal Space Ivar Østby1,2., Leiv Øyehaug1,2., Gaute T. Einevoll1,2, Erlend A. Nagelhus3,4, Erik Plahte1,2, Thomas 4,5 6 3,4 1,7 Zeuthen , Catherine M. Lloyd , Ole P. Ottersen , Stig W. Omholt * ˚ 1 Centre for Integrative Genetics (CIGENE), Norwegian University of Life Sciences, As, Norway, 2 Department of Mathematical Sciences and Technology, Norwegian ˚ University of Life Sciences, As, Norway, 3 Centre for Molecular Biology and Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Blindern, Oslo, Norway, 4 Nordic Centre for Water Imbalance Related Disorders, University of Oslo, Blindern, Oslo, Norway, 5 Institute of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark, 6 Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand, 7 Department of Animal and ˚ Aquacultural Sciences, Norwegian University of Life Sciences, As, Norway Abstract Neuronal stimulation causes ,30% shrinkage of the extracellular space (ECS) between neurons and surrounding astrocytes in grey and white matter under experimental conditions. Despite its possible implications for a proper understanding of basic aspects of potassium clearance and astrocyte function, the phenomenon remains unexplained. Here we present a dynamic model that accounts for current experimental data related to the shrinkage phenomenon in wild-type as well as in gene knockout individuals. We find that neuronal release of potassium and uptake of sodium during stimu