cardiovascular agents affect the tone of pulmonary arteries and veins in precision-cut lung slices心血管药物影响肺的动脉和静脉的基调展示肺片.pdfVIP

Cardiovascular Agents Affect the Tone of Pulmonary Arteries and Veins in Precision-Cut Lung Slices 1,2 1 2 2 Annette D. Rieg *, Rolf Rossaint , Stefan Uhlig , Christian Martin 1 Department of Anaesthesiology, Institute of Pharmacology and Toxicology, Medical Faculty Aachen, RWTH-Aachen, Aachen, Germany, 2 Institute of Pharmacology and Toxicology, Medical Faculty Aachen, RWTH-Aachen, Aachen, Germany Abstract Introduction: Cardiovascular agents are pivotal in the therapy of heart failure. Apart from their action on ventricular contractility and systemic afterload, they affect pulmonary arteries and veins. Although these effects are crucial in heart failure with coexisting pulmonary hypertension or lung oedema, they are poorly defined, especially in pulmonary veins. Therefore, we investigated the pulmonary vascular effects of adrenoceptor agonists, vasopressin and angiotensin II in the model of precision-cut lung slices that allows simultaneous studies of pulmonary arteries and veins. Materials and Methods: Precision-cut lung slices were prepared from guinea pigs and imaged by videomicroscopy. Concentration-response curves of cardiovascular drugs were analysed in pulmonary arteries and veins. Results: Pulmonary veins responded stronger than arteries to a -agonists (contraction) and b -agonists (relaxation). 1 2 Notably, inhibition of b -adrenoceptors unmasked the a -mimetic effect of norepinephrine and epinephrine in pulmonary 2 1 veins. Vasopressin and angiotensin II contracted pulmonary veins via V1a and AT1