branched chain fatty acids reduce the incidence of necrotizing enterocolitis and alter gastrointestinal microbial ecology in a neonatal rat model支链脂肪酸减少的发生率和改变胃肠道微生物生态学新生儿坏死性小肠结肠炎大鼠模型.pdfVIP

Branched Chain Fatty Acids Reduce the Incidence of Necrotizing Enterocolitis and Alter Gastrointestinal Microbial Ecology in a Neonatal Rat Model 1 2 2 2 Rinat R. Ran-Ressler , Ludmila Khailova , Kelly M. Arganbright , Camille K. Adkins-Rieck , Zeina E. 3 4 4 1 2 Jouni , Omry Koren , Ruth E. Ley , J. Thomas Brenna *, Bohuslav Dvorak * 1 Division of Nutritional Sciences, Savage Hall, Cornell University, Ithaca, New York, United States of America, 2 Department of Pediatrics, University of Arizona, Tucson, Arizona, United States of America, 3 Mead Johnson Nutrition, Evansville, Indiana, United States of America, 4 Department of Microbiology, Cornell University, Ithaca, New York, United States of America Abstract Introduction: Branched chain fatty acids (BCFA) are found in the normal term human newborn’s gut, deposited as major components of vernix caseosa ingested during late fetal life. We tested the hypothesis that premature infants’ lack of exposure to gastrointestinal (GI) BCFA is associated with their microbiota and risk for necrotizing enterocolitis (NEC) using a neonatal rat model. Methods: Pups were collected one day before scheduled birth. The pups were exposed to asphyxia and cold stress to induce NEC. Pups were assigned to one of three experimental treatments. DF (dam-fed) ; Control, hand-fed rat milk substitute ; BCFA, hand-fed rat milk substitute with 20%w/w BCFA. Total fat was equivalent (11%wt) for both the Control and BCFA groups. Cecal microbiota were characterized by 16S rRNA gene pyrosequencing, and intestinal injury, ileal cytokine and mucin gene expression, i