beyond streptococcus mutans dental caries onset linked to multiple species by 16s rrna community analysis除了变形链球菌龋齿发病与多个物种16 s rrna社区分析.pdfVIP

Beyond Streptococcus mutans: Dental Caries Onset Linked to Multiple Species by 16S rRNA Community Analysis 1 1 2,3 1 1 2,3 Erin L. Gross , Clifford J. Beall , Stacey R. Kutsch , Noah D. Firestone , Eugene J. Leys , Ann L. Griffen * 1 Division of Oral Biology, College of Dentistry, The Ohio State University, Columbus, Ohio, United States of America, 2 Division of Pediatric Dentistry and Community Oral Health, College of Dentistry, The Ohio State University, Columbus, Ohio, United States of America, 3 Department of Dentistry, Nationwide Children’s Hospital, Columbus, Ohio, United States of America Abstract Dental caries in very young children may be severe, result in serious infection, and require general anesthesia for treatment. Dental caries results from a shift within the biofilm community specific to the tooth surface, and acidogenic species are responsible for caries. Streptococcus mutans, the most common acid producer in caries, is not always present and occurs as part of a complex microbial community. Understanding the degree to which multiple acidogenic species provide functional redundancy and resilience to caries-associated communities will be important for developing biologic interventions. In addition, microbial community interactions in health and caries pathogenesis are not well understood. The purpose of this study was to investigate bacterial community profiles associated with the onset of caries in the primary dentition. In a combination cross-sectional and longitudinal design, bacterial community profiles at progressive stages of caries and over time were examined and compared to those of health. 16S rRNA gene sequencing was used for bact