atlas of signaling for interpretation of microarray experiments阿特拉斯的信号对微阵列实验的理解.pdfVIP

Atlas of Signaling for Interpretation of Microarray Experiments . . Ekaterina Kotelnikova , Natalia Ivanikova , Andrey Kalinin, Anton Yuryev, Nikolai Daraselia* Ariadne Genomics, Inc., Rockville, Maryland, United States of America Abstract Microarray-based expression profiling of living systems is a quick and inexpensive method to obtain insights into the nature of various diseases and phenotypes. A typical microarray profile can yield hundreds or even thousands of differentially expressed genes and finding biologically plausible themes or regulatory mechanisms underlying these changes is a non- trivial and daunting task. We describe a novel approach for systems-level interpretation of microarray expression data using a manually constructed ‘‘overview’’ pathway depicting the main cellular signaling channels (Atlas of Signaling). Currently, the developed pathway focuses on signal transduction from surface receptors to transcription factors and further transcriptional regulation of cellular ‘‘workhorse’’ proteins. We show how the constructed Atlas of Signaling in combination with an enrichment analysis algorithm allows quick identification and visualization of the main signaling cascades and cellular processes affected in a gene expression profiling experiment. We validate our approach using several publicly available gene expression datasets. Citation: Kotelnikova E, Ivanikova N, Kalinin A, Yuryev A, Daraselia N (2010) Atlas of Signaling for Interpretation of Microarray Experiments. PLoS ONE 5(2): e9256. doi:10.1371/journal.pone.0009256 Editor: I. King Jordan, Georgia Institute of Technology, United States of America Received October 12, 2009; Accepted January 22, 2010; Published February 17, 2010 Co