arterially perfused neurosphere-derived cells distribute outside the ischemic core in a model of transient focal ischemia and reperfusion in vitro动脉灌注neurosphere-derived细胞分布在缺血性核心模型的瞬态局部缺血和再灌注体外.pdfVIP

Arterially Perfused Neurosphere-Derived Cells Distribute Outside the Ischemic Core in a Model of Transient Focal Ischemia and Reperfusion In Vitro 1,5 1 1 1 1 Chiara Pastori , Laura Librizzi , Gian Luca Breschi , Cristina Regondi , Carolina Frassoni , Ferruccio 2 3 3 3 4 Panzica , Simona Frigerio , Maurizio Gelati , Eugenio Parati , Maria Grazia De Simoni , Marco de Curtis1* ` ` 1 Unita di Neurofisiologia ed Epilettologia Sperimentale, Fondazione Istituto Neurologico Carlo Besta, Milan, Italy, 2 Unita di Neurofisiopatologia ed Epilettologia diagnostica, Fondazione Istituto Neurologico Carlo Besta, Milan, Italy, 3 SDS Neurobiologia e Terapie Neuroriparative, Fondazione Istituto Neurologico Carlo Besta, Milan, Italy, 4 Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy, 5 Neurology Residency School, University of Milano-Bicocca, Monza, Italy Abstract Background: Treatment with neural stem cells represents a potential strategy to improve functional recovery of post- ischemic cerebral injury. The potential benefit of such treatment in acute phases of human ischemic stroke depends on the therapeutic viability of a systemic vascular delivery route. In spite of the large number of reports on the beneficial effects of intracerebral stem cells injection in experimental stroke, very few studies demonstrated the effectiveness of the systemic intravenous delivery approach. Metodology/Principal Findings: We utilized a novel in vitro model of transient focal ische